DEVELOPMENT OF MICROSATELLITE MARKERS USING ILLUMINA MISEQ SEQUENCING TO CHARACTERIZE EPHEDRA GERARDIANA (EPHEDRACEAE)

被引：6

作者：

De, Ji ^{[1
,2
]}

Zhu, Weidong ^{[2
]}

Liu, Tianmeng ^{[2
]}

Wang, Zhe ^{[1
]}

Zhong, Yang ^{[1
,2
]}

机构：

[1] Fudan Univ, Sch Life Sci, Shanghai 200438, Peoples R China

[2] Tibet Univ, Sci Fac, Lhasa 850000, Tibet Autonomou, Peoples R China

来源：

APPLICATIONS IN PLANT SCIENCES | 2017年 / 5卷 / 03期

基金：

中国国家自然科学基金;

关键词：

conservation; Ephedra gerardiana; Ephedraceae; genetic diversity; next-generation sequencing; simple sequence repeat markers; DATABASES;

D O I：

10.3732/apps.1600104

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Ephedra gerardiana (Ephedraceae), occurring in the Himalayan ranges, is an important plant species used in Tibetan medicine. Due to the lack of molecular markers to characterize genetic diversity, knowledge for conservation and uses of E. gerardiana resources is limited; we therefore developed microsatellite markers for use in this species. Methods and Results: Using Illumina MiSeq sequencing technology, we developed 29 polymorphic microsatellite loci suitable for E. gerardiana, of which 15 loci also showed polymorphisms in two related Ephedra species, E. saxatilis and E. monosperma. The average number of effective alleles per locus ranged from two to six. The observed and expected heterozygosity ranged from 0.23 to 0.83 and 0.44 to 0.86, respectively, in E. gerardiana populations. Conclusions: The developed 29 microsatellite markers are effective for the study of genetic structure and genetic diversity of E. gerardiana, and 15 of these markers are suitable for related Ephedra species.

引用

页数：4

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