Efficient delivery of lentiviral vectors into resting human CD4 T cells

被引：25

作者：

Geng, X. ^{[1
]}

Doitsh, G. ^{[1
]}

Yang, Z. ^{[1
]}

Galloway, N. L. K. ^{[1
]}

Greene, W. C. ^{[1
,2
,3
]}

机构：

[1] J David Gladstone Inst, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Dept Med, San Francisco, CA USA

[3] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

来源：

GENE THERAPY | 2014年 / 21卷 / 04期

关键词：

lentivirus; CD4 T cells; shRNA; Vpx; HIV; AIDS; IMMUNODEFICIENCY-VIRUS TYPE-1; HIV-1; INFECTION; DENDRITIC CELLS; GENE-TRANSFER; SAMHD1; TRANSDUCTION; TRANSCRIPTION; LYMPHOCYTES; INHIBITION; SUBSETS;

D O I：

10.1038/gt.2014.5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.

引用

收藏

页码：444 / 449

页数：6

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