Efficient delivery of lentiviral vectors into resting human CD4 T cells

被引：24

作者：

Geng, X. ^{[1
]}

Doitsh, G. ^{[1
]}

Yang, Z. ^{[1
]}

Galloway, N. L. K. ^{[1
]}

Greene, W. C. ^{[1
,2
,3
]}

机构：

[1] J David Gladstone Inst, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Dept Med, San Francisco, CA USA

[3] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

来源：

GENE THERAPY | 2014年 / 21卷 / 04期

关键词：

lentivirus; CD4 T cells; shRNA; Vpx; HIV; AIDS; IMMUNODEFICIENCY-VIRUS TYPE-1; HIV-1; INFECTION; DENDRITIC CELLS; GENE-TRANSFER; SAMHD1; TRANSDUCTION; TRANSCRIPTION; LYMPHOCYTES; INHIBITION; SUBSETS;

D O I：

10.1038/gt.2014.5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.

引用

收藏

页码：444 / 449

页数：6

相关论文

共 45 条

[1] The CXCR4-Tropic Human Immunodeficiency Virus Envelope Promotes More-Efficient Gene Delivery to Resting CD4+ T Cells than the Vesicular Stomatitis Virus Glycoprotein G Envelope [J].

Agosto, Luis M. ;

Yu, Jianqing J. ;

Liszewski, Megan K. ;

Baytop, Clifford ;

Korokhov, Nikolay ;

Humeau, Laurent M. ;

O'Doherty, Una .

JOURNAL OF VIROLOGY, 2009, 83 (16) :8153-8162

[2] SAMHD1 restricts HIV-1 infection in resting CD4+ T cells [J].

Baldauf, Hanna-Mari ;

Pan, Xiaoyu ;

Erikson, Elina ;

Schmidt, Sarah ;

Daddacha, Waaqo ;

Burggraf, Manja ;

Schenkova, Kristina ;

Ambiel, Ina ;

Wabnitz, Guido ;

Gramberg, Thomas ;

Panitz, Sylvia ;

Flory, Egbert ;

Landau, Nathaniel R. ;

Sertel, Serkan ;

Rutsch, Frank ;

Lasitschka, Felix ;

Kim, Baek ;

Koenig, Renate ;

Fackler, Oliver T. ;

Keppler, Oliver T. .

NATURE MEDICINE, 2012, 18 (11) :1682-+

[3] A simple, versatile and efficient method to genetically modify human monocyte-derived dendritic cells with HIV-1-derived lentiviral vectors [J].

Berger, Gregory ;

Durand, Stephanie ;

Goujon, Caroline ;

Xuan-Nhi Nguyen ;

Cordeil, Stephanie ;

Darlix, Jean-Luc ;

Cimarelli, Andrea .

NATURE PROTOCOLS, 2011, 6 (06) :806-816

[4] Efficient transduction of myeloid cells by an HIV-1-derived lentiviral vector that packages the Vpx accessory protein [J].

Bobadilla, S. ;

Sunseri, N. ;

Landau, N. R. .

GENE THERAPY, 2013, 20 (05) :514-520

[5] A sensitive and specific enzyme-based assay detecting HIV-1 virion fusion in primary T lymphocytes [J].

Cavrois, M ;

de Noronha, C ;

Greene, WC .

NATURE BIOTECHNOLOGY, 2002, 20 (11) :1151-1154

[6] Human Immunodeficiency Virus Integrates Directly into Naive Resting CD4+ T Cells but Enters Naive Cells Less Efficiently than Memory Cells [J].

Dai, Jihong ;

Agosto, Luis M. ;

Baytop, Clifford ;

Yu, Jianqing J. ;

Pace, Matthew J. ;

Liszewski, Megan K. ;

O'Doherty, Una .

JOURNAL OF VIROLOGY, 2009, 83 (09) :4528-4537

[7] Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques [J].

Derdouch, Sonia ;

Gay, Wilfried ;

Negre, Didier ;

Prost, Stephane ;

Le Dantec, Mikael ;

Delache, Benoit ;

Auregan, Gwenaelle ;

Andrieu, Thibault ;

Leplat, Jean-Jacques ;

Cosset, Francois-Loic ;

Le Grand, Roger .

RETROVIROLOGY, 2008, 5 (1)

[8] Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 infection [J].

Doitsh, Gilad ;

Galloway, Nicole L. K. ;

Geng, Xin ;

Yang, Zhiyuan ;

Monroe, Kathryn M. ;

Zepeda, Orlando ;

Hunt, Peter W. ;

Hatano, Hiroyu ;

Sowinski, Stefanie ;

Munoz-Arias, Isa ;

Greene, Warner C. .

NATURE, 2014, 505 (7484) :509-+

[9] Abortive HIV Infection Mediates CD4 T Cell Depletion and Inflammation in Human Lymphoid Tissue [J].

Doitsh, Gilad ;

Cavrois, Marielle ;

Lassen, Kara G. ;

Zepeda, Orlando ;

Yang, Zhiyuan ;

Santiago, Mario L. ;

Hebbeler, Andrew M. ;

Greene, Warner C. .

CELL, 2010, 143 (05) :789-801

[10] BDCA-2, BDCA-3 and BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood [J].

Dzionek, A ;

Fuchs, A ;

Schmidt, P ;

Cremer, S ;

Zysk, M ;

Miltenyi, S ;

Buck, DW ;

Schmitz, J .

JOURNAL OF IMMUNOLOGY, 2000, 165 (11) :6037-6046

← 1 2 3 4 5 →