A highly sensitive near-infrared ratiometric fluorescent probe for detecting nitroreductase and cellular imaging

被引：56

作者：

Zhu, Dongjian ^{[1
,2
]}

Xue, Lin ^{[2
]}

Li, Guoping ^{[2
]}

Jiang, Hua ^{[1
]}

机构：

[1] Beijing Normal Univ, Coll Chem, Minist Educ, Key Lab Radiopharmaceut, Beijing 100875, Peoples R China

[2] Chinese Acad Sci, Inst Chem, CAS Key Lab Photochem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 222卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Near-infrared; Ratiometric; Nitroreductase; Cellular imaging; TUMOR HYPOXIA; CANCER-TREATMENT; AQUEOUS-SOLUTION; CELLS; OXYGEN; PIMONIDAZOLE; CONJUGATE; CARCINOMA; FLUOROPHORE; ACTIVATION;

D O I：

10.1016/j.snb.2015.08.093

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on the selective reduction of nitro group to hydroxylamine or amino group by nitroreductase in the presence of NADPH as an electron donor, a near-infrared ratiometric fluorescent probe CyNNO2 was designed and synthesized. CyNNO2 containing a p-nitrobenzyl moiety as a reactive group can selectively respond to nitroreductase with a ratiometric fluorescence signal output. The limit of detection for nitroreductase is 0.0058 ng/mL. Moreover, the probe can be used to image the endogenous nitroreductase in A549 cells under hypoxic condition. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：419 / 424

页数：6

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