A highly sensitive near-infrared ratiometric fluorescent probe for detecting nitroreductase and cellular imaging

被引：56
作者：
Zhu, Dongjian [1 ,2 ]
Xue, Lin [2 ]
Li, Guoping [2 ]
Jiang, Hua [1 ]
机构：
[1] Beijing Normal Univ, Coll Chem, Minist Educ, Key Lab Radiopharmaceut, Beijing 100875, Peoples R China
[2] Chinese Acad Sci, Inst Chem, CAS Key Lab Photochem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 222卷
基金：
中国国家自然科学基金;
关键词：
Fluorescent probe; Near-infrared; Ratiometric; Nitroreductase; Cellular imaging; TUMOR HYPOXIA; CANCER-TREATMENT; AQUEOUS-SOLUTION; CELLS; OXYGEN; PIMONIDAZOLE; CONJUGATE; CARCINOMA; FLUOROPHORE; ACTIVATION;
D O I：
10.1016/j.snb.2015.08.093
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Based on the selective reduction of nitro group to hydroxylamine or amino group by nitroreductase in the presence of NADPH as an electron donor, a near-infrared ratiometric fluorescent probe CyNNO2 was designed and synthesized. CyNNO2 containing a p-nitrobenzyl moiety as a reactive group can selectively respond to nitroreductase with a ratiometric fluorescence signal output. The limit of detection for nitroreductase is 0.0058 ng/mL. Moreover, the probe can be used to image the endogenous nitroreductase in A549 cells under hypoxic condition. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：419 / 424
页数：6
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