A highly sensitive near-infrared ratiometric fluorescent probe for detecting nitroreductase and cellular imaging

被引：56
作者：
Zhu, Dongjian [1 ,2 ]
Xue, Lin [2 ]
Li, Guoping [2 ]
Jiang, Hua [1 ]
机构：
[1] Beijing Normal Univ, Coll Chem, Minist Educ, Key Lab Radiopharmaceut, Beijing 100875, Peoples R China
[2] Chinese Acad Sci, Inst Chem, CAS Key Lab Photochem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 222卷
基金：
中国国家自然科学基金;
关键词：
Fluorescent probe; Near-infrared; Ratiometric; Nitroreductase; Cellular imaging; TUMOR HYPOXIA; CANCER-TREATMENT; AQUEOUS-SOLUTION; CELLS; OXYGEN; PIMONIDAZOLE; CONJUGATE; CARCINOMA; FLUOROPHORE; ACTIVATION;
D O I：
10.1016/j.snb.2015.08.093
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Based on the selective reduction of nitro group to hydroxylamine or amino group by nitroreductase in the presence of NADPH as an electron donor, a near-infrared ratiometric fluorescent probe CyNNO2 was designed and synthesized. CyNNO2 containing a p-nitrobenzyl moiety as a reactive group can selectively respond to nitroreductase with a ratiometric fluorescence signal output. The limit of detection for nitroreductase is 0.0058 ng/mL. Moreover, the probe can be used to image the endogenous nitroreductase in A549 cells under hypoxic condition. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：419 / 424
页数：6
相关论文
共 50 条
  • [1] A highly sensitive near-infrared ratiometric fluorescent probe for imaging of mitochondrial hydrazine in cells and in mice models
    Song, Yaqun
    Chen, Guang
    Han, Xiaoyue
    You, Jinmao
    Yu, Fabiao
    SENSORS AND ACTUATORS B-CHEMICAL, 2019, 286 : 69 - 76
  • [2] A highly sensitive and selective near-infrared fluorescent probe for imaging hydrazine in living tissues and mice
    Wang, Shen
    Ma, Siyue
    Zhang, Jidong
    She, Mengyao
    Liu, Ping
    Zhang, Shengyong
    Li, Jianli
    SENSORS AND ACTUATORS B-CHEMICAL, 2018, 261 : 418 - 424
  • [3] A near-infrared fluorescent probe for ratiometric imaging peroxynitrite in Parkinson's disease modelY
    Kang, Hao
    Shu, Wei
    Yu, Jin
    Gao, Mengxu
    Han, Rubing
    Jing, Jing
    Zhang, Rubo
    Zhang, Xiaoling
    SENSORS AND ACTUATORS B-CHEMICAL, 2022, 359
  • [4] Near-Infrared Ratiometric Fluorescent Probe for Detecting Endogenous Cu2+ in the Brain
    Zhu, Jianping
    Graziotto, Marcus E.
    Cottam, Veronica
    Hawtrey, Tom
    Adair, Liam D.
    Trist, Benjamin G.
    Pham, Nguyen T. H.
    Rouaen, Jourdin R. C.
    Ohno, Carolyn
    Heisler, Marcus
    Vittorio, Orazio
    Double, Kay L.
    New, Elizabeth J.
    ACS SENSORS, 2024, 9 (06): : 2858 - 2868
  • [5] A Highly Sensitive and Selective Near-Infrared Fluorescent Probe for Detecting Peroxynitrite in Living Cells and Drosophila Brains
    Wang, Wei
    Deng, Jian-Bin
    Jin, Long
    Guan, Bai-Ou
    CHEMOSENSORS, 2023, 11 (05)
  • [6] A Highly Sensitive Chemiluminescent Probe for Detecting Nitroreductase and Imaging in Living Animals
    Sun, Jinyu
    Hu, Zhian
    Wang, Ruihua
    Zhang, Sichun
    Zhang, Xinrong
    ANALYTICAL CHEMISTRY, 2019, 91 (02) : 1384 - 1390
  • [7] A near-infrared fluorescent probe for the ratiometric detection and living cell imaging of β-galactosidase
    Zhang, Xueyan
    Chen, Xiangzhu
    Zhang, Yuanyuan
    Liu, Kaizheng
    Shen, Hongjing
    Zheng, En
    Huang, Xiaoqian
    Hou, Shicong
    Ma, Xiaodong
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2019, 411 (30) : 7957 - 7966
  • [8] A near-infrared fluorescent probe for the ratiometric detection and living cell imaging of β-galactosidase
    Xueyan Zhang
    Xiangzhu Chen
    Yuanyuan Zhang
    Kaizheng Liu
    Hongjing Shen
    En Zheng
    Xiaoqian Huang
    Shicong Hou
    Xiaodong Ma
    Analytical and Bioanalytical Chemistry, 2019, 411 : 7957 - 7966
  • [9] A ratiometric near-infrared naphthalimide-based fluorescent probe with high sensitivity for detecting Fe2+ in vivo
    Huang, Lina
    Chen, Yu
    Zhao, Yuqiang
    Wang, Yumin
    Xiong, Junwei
    Zhang, Junfeng
    Wu, Xianghua
    Zhou, Ying
    CHINESE CHEMICAL LETTERS, 2020, 31 (11) : 2941 - 2944
  • [10] A novel near-infrared ratiometric fluorescent probe targeting lysosomes for imaging HOCl in vitro and in vivo
    Chen, Xiong
    Jiang, Detao
    Jiang, Chen
    Yao, Cheng
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2023, 286
12345