Effect of Probe Design on Chemiluminescence Detection of Hepatitis B Virus and Hepatitis C Virus

被引：7

作者：

Ali, Zeeshan ^{[1
,2
,3
]}

Tang, Yongjun ^{[1
]}

Deng, Yan ^{[4
,5
]}

Li, Hanmei ^{[1
]}

Wang, Zhifei ^{[3
]}

Li, Zhiyang ^{[2
]}

Dai, Jianguo ^{[1
]}

Liu, Hongna ^{[4
,5
]}

Su, Enben ^{[2
,6
]}

Chen, Zhu ^{[4
,5
]}

Li, Song ^{[4
,5
]}

Chen, Hui ^{[4
,5
]}

Wang, Lijun ^{[7
,8
,9
]}

机构：

[1] Shenzhen Polytech, Postdoctoral Innovat Practice Base, Shenzhen 518055, Peoples R China

[2] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

[3] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China

[4] Econ Forest Cultivat & Utilizat 2011 Collaborat I, Zhuzhou 412007, Peoples R China

[5] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Zhuzhou 412007, Peoples R China

[6] Getein Biotechnol Co Ltd, Nanjing 211505, Jiangsu, Peoples R China

[7] Guangxi Univ, Guangxi Key Lab Clean Pulp & Paper Making & Pollu, Nanning 530004, Peoples R China

[8] Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China

[9] Collaborat Innovat Ctr Guangxi Sugar Ind, Nanning 530004, Peoples R China

来源：

NANOSCIENCE AND NANOTECHNOLOGY LETTERS | 2018年 / 10卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Probe Design; Hepatitis B Virus (HBV); Hepatitis C Virus (HCV); Chemiluminescence; Biosensor; POLYMERASE-CHAIN-REACTION; BOVINE SERUM-ALBUMIN; NUMBER VARIATION ANALYSIS; MAGNETIC NANOPARTICLES; RAPID DETECTION; GOLD NANORODS; STEM-CELLS; ACETYLCHOLINESTERASE BIOSENSOR; ISOTHERMAL AMPLIFICATION; ULTRASENSITIVE DETECTION;

D O I：

10.1166/nnl.2018.2585

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Probe design is an important part in fabricating highly sensitive bioassays. In this paper, it is demonstrated that even small changes in probe design parameters can scupper efficiency of any biosensor. Different probes were designed for capturing HBV, and HCV biotin-labeled PCR amplified segments in a chemiluminescence detection assay. It was found that probes binding to the terminal sequence of target DNA generated strong detection signals; however, specificity of these probes was poor. Probe length was also noticed to play a critical role in the successful probetarget bio-recognition event. Probes with sequence length equal to 18 bp or higher outstripped the probes having sequence length shorter than 18 bp. Similarly, Probes with higher GC contents performed better in comparison with probes with lower GC contents. Conclusively, these results could be helpful in making highly efficient biosensors in future.

引用

页码：127 / 134

页数：8