Expression and localization of Aquaporin 3 (AQP3) in folliculogenesis of ewes

被引：11

作者：

Sales, Antonia Debora ^{[1
]}

Brito, Ivina Rocha ^{[1
]}

de Lima, Laritza Ferreira ^{[1
]}

Lobo, Carlos Henrique ^{[2
]}

Graca Duarte, Ana Beatriz ^{[1
]}

Azevedo Souza, Carlos Eduardo ^{[2
]}

Moura, Arlindo Alencar ^{[2
]}

de Figueiredo, Jose Ricardo ^{[1
]}

Ribeiro Rodrigues, Ana Paula ^{[1
]}

机构：

[1] Univ Estadual Ceara, Oocytes & Ovarian Preantral Follicles Lab LAMOFOP, Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, Dept Anim Sci, Biol Reprod Res Grp, Fortaleza, Ceara, Brazil

来源：

ACTA HISTOCHEMICA | 2014年 / 116卷 / 05期

关键词：

Aquaporin; 3; Ovarian follicles; Sheep; FOLLICULAR-GROWTH; WATER CHANNELS; GENES; OOCYTES; PROTEIN; CYCLE; TIME;

D O I：

10.1016/j.acthis.2014.02.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The mRNA expression and localization of Aquaporin 3 (AQP3) were investigated in the ovarian follicles of ewes at different stages of development (primordial, primary, secondary, small, and large antral). The gene expression was quantified by qPCR, while the protein identification and localization were determined by Western blot and immunohistochemistry, respectively. Analysis revealed that AQP3 mRNA was detected only in the antral follicles, whereas the protein expression was detected in the oocyte and granulosa cells in all stages of follicular development. The latter observation suggests that the presence of AQP3 in follicles of all categories, especially in the antral follicles, provides novel insights on the mechanisms that regulate the flow of water between cells during the formation of antral follicles in sheep. (C) 2014 Elsevier GmbH. All rights reserved.

引用

页码：831 / 837

页数：7

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