Thyroid hormone (T3) is involved in inhibiting the proliferation of newborn calf Sertoli cells via the PI3K/Akt signaling pathway in vitro

The experiment was designed to study the effects of Thyroid hormone (T-3) on the proliferation and differentiation of newborn calf Sertoli cells (SCs) to provide a theoretical and practical basis for increased testicular semen production. In this experiment, the cck8 method was used to detect the effects of different concentrations of T-3 on the proliferation rate of newborn calf SCs. qPCR and Western Blot methods were used to explore the effects of T-3 on the proliferation and differentiation of calves SCs and whether T-3 through Wnt/beta-catenin and PI3K/Akt pathways can regulate the proliferation and differentiation of SCs. We found that dosage (T-3) and time correlated with proliferation inhibition of SC. T-3 inhibited the proliferation of SC by down-regulating cyclinD1, upregulating p21Cip, p27Kip1, and other cell-cycle factors. By up-regulating AR and down-regulating KRT-18, T-3 promoted the maturated differentiation of SC. T-3 could not affect the expression of beta-catenin in SC of newborn calf, indicating that T-3 may not regulate SCs proliferation through the Wnt pathway. T-3 also negatively regulated the gene expression and protein levels of some genes in the PI3K/Akt signaling pathway. We concluded that T-3 inhibited newborn calf SCs proliferation through the PI3K/Akt signaling pathway and possibly promoted their differentiation. (C) 2019 Elsevier Inc. All rights reserved.