Preparation of size tunable giant vesicles from cross-linked dextran(ethylene glycol) hydrogels

被引:50
作者
Mora, Nestor Lopez [1 ]
Hansen, Jesper S. [2 ]
Gao, Yue [1 ]
Ronald, Andrew A. [2 ]
Kieltyka, Roxanne [1 ]
Malmstadt, Noah [2 ]
Kros, Alexander [1 ]
机构
[1] Leiden Univ, Leiden Inst Chem, NL-2300 RA Leiden, Netherlands
[2] Univ So Calif, Mork Family Dept Chem Engn & Mat Sci, Los Angeles, CA 90007 USA
基金
欧洲研究理事会;
关键词
UNILAMELLAR VESICLES; MEMBRANE-FUSION; PHYSIOLOGICAL CONDITIONS; LIPOSOMES; FILMS;
D O I
10.1039/c3cc49144g
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We present a novel chemically cross-linked dextran-poly(ethylene glycol) hydrogel substrate for the preparation of dense vesicle suspensions under physiological ionic strength conditions. These vesicles can be easily diluted for individual study. Modulating the degree of cross-linking within the hydrogel network results in tuning of the vesicle size distribution.
引用
收藏
页码:1953 / 1955
页数:3
相关论文
共 21 条
[1]   Preparation of giant liposomes in physiological conditions and their characterization under an optical microscope [J].
Akashi, K ;
Miyata, H ;
Itoh, H ;
Kinosita, K .
BIOPHYSICAL JOURNAL, 1996, 71 (06) :3242-3250
[2]   Formation of giant liposomes promoted by divalent cations: Critical role of electrostatic repulsion [J].
Akashi, K ;
Miyata, H ;
Itoh, H ;
Kinosita, K .
BIOPHYSICAL JOURNAL, 1998, 74 (06) :2973-2982
[3]   LIPOSOME ELECTROFORMATION [J].
ANGELOVA, MI ;
DIMITROV, DS .
FARADAY DISCUSSIONS, 1986, 81 :303-+
[4]   Films of Agarose Enable Rapid Formation of Giant Liposomes in Solutions of Physiologic Ionic Strength [J].
Horger, Kim S. ;
Estes, Daniel J. ;
Capone, Ricardo ;
Mayer, Michael .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (05) :1810-1819
[5]   A Reduced SNARE Model for Membrane Fusion [J].
Marsden, Hana Robson ;
Elbers, Nina A. ;
Bomans, Paid H. H. ;
Sommerdijk, Nico A. J. M. ;
Kros, Alexander .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (13) :2330-2333
[6]   Giant unilamellar vesicles electroformed from native membranes and organic lipid mixtures under physiological conditions [J].
Montes, L.-Ruth ;
Alonso, Alicia ;
Goni, Felix M. ;
Bagatolli, Luis A. .
BIOPHYSICAL JOURNAL, 2007, 93 (10) :3548-3554
[7]   GUV preparation and imaging: Minimizing artifacts [J].
Morales-Penningston, Nelson F. ;
Wu, Jing ;
Farkas, Elaine R. ;
Goh, Shih Lin ;
Konyakhina, Tatyana M. ;
Zheng, Judy Y. ;
Webb, Watt W. ;
Feigenson, Gerald W. .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2010, 1798 (07) :1324-1332
[8]   Size control of giant unilamellar vesicles prepared from inverted emulsion droplets [J].
Nishimura, Kazuya ;
Suzuki, Hiroaki ;
Toyota, Taro ;
Yomo, Tetsuya .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2012, 376 :119-125
[9]   Changes in the morphology of cell-size liposomes in the presence of cholesterol: Formation of neuron-like tubes and liposome networks [J].
Nomura, SM ;
Mizutani, Y ;
Kurita, K ;
Watanabe, A ;
Akiyoshi, K .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2005, 1669 (02) :164-169
[10]   Dextran based photodegradable hydrogels formed via a Michael addition [J].
Peng, Ke ;
Tomatsu, Itsuro ;
van den Broek, Bram ;
Cui, Chao ;
Korobko, Alexander V. ;
van Noort, John ;
Meijer, Annemarie H. ;
Spaink, Herman P. ;
Kros, Alexander .
SOFT MATTER, 2011, 7 (10) :4881-4887