CircRNA hsa_circ_0004771 promotes esophageal squamous cell cancer progression via miR-339-5p/CDC25A axis

被引：61

作者：

Huang, Enmin ^{[1
]}

Fu, Junhui ^{[2
]}

Yu, Qiuyan ^{[3
]}

Xie, Peixin ^{[2
]}

Yang, Zhongxian ^{[4
]}

Ji, Huanlin ^{[3
]}

Wang, Li ^{[3
]}

Luo, Ganfeng ^{[3
]}

Zhang, Yanting ^{[3
]}

Li, Ke ^{[3
]}

机构：

[1] Shantou Univ, Dept Gen Surg, Affiliated Hosp 2, Med Coll, Shantou, Guangdong, Peoples R China

[2] Shantou Cent Hosp, Dept Surg Oncol, Shantou, Guangdong, Peoples R China

[3] Shantou Univ, Dept Publ Hlth, Med Coll, Shantou, Guangdong, Peoples R China

[4] Shantou Univ, Dept Med Imaging, Affiliated Hosp 2, Med Coll, Shantou, Guangdong, Peoples R China

来源：

EPIGENOMICS | 2020年 / 12卷 / 07期

基金：

中国国家自然科学基金;

关键词：

CDC25A; circRNA; differential expression; ESCC; hsa_circ_0004771; mechanism study; miR-339-5p; molecular sponge; RNA-Seq analysis; tumor biomarker; CIRCULAR RNA; TUMOR-SUPPRESSOR; CARCINOMA; MICRORNA; IDENTIFICATION; CDC25A; EXPRESSION; BIOMARKER; EFFICIENT; EXOSOMES;

D O I：

10.2217/epi-2019-0404

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: The role of circRNAs in esophageal squamous cell cancer (ESCC) remains unclear. Materials & methods: Here we profiled six pair plasma circRNA in ESCC based on RNA sequencing, and then verified the elevation of hsa_circ_0004771 in 20 cancer tissues and 105 pair case-control plasma samples by quantitative reverse transcriptase PCR. Results: The upregulation of hsa_circ_0004771 was correlated with heavier tumor burden and poor prognosis, knockdown of it inhibited the ESCC cells proliferation both in vitro and in vivo. Mechanistically, hsa_circ_0004771 positively regulated CDC25A by acting as a molecular sponge of miR-339-5p and rescue assay confirmed this regulatory relationship. Conclusion: These results suggested that hsa_circ_0004771 can serve as a general less-invasive biomarker and may provide diagnostic and prognostic value in carcinoma.

引用

页码：587 / 603

页数：17

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