Effects of Lycium barbarum polysaccharides on expression of hypoxia inducible factor 1α and vascular endothelial growth factor in human immortalized keratinocytes cells irradiated by ultraviolet B

Studies have shown that LBP (Lycium barbarum Polysaccharides) can protect the HaCaT (Human Immortalized keratinocytes cells) against the damage caused by UVB (Ultraviolet B). To probe the effects of LBP on the HIF-1 alpha and VEGF from HaCaT cells irradiated by UVB, HaCaT was disposed of LBP following by irradiating with different energy density of UVB for 24 h. The cell viability and activity of SOD (Super Oxide Dismutase) was measured by CCK-8 and enzyme-linked immunoassay, respectively. RT-PCR and western blot were employed to study the mRNA and protein expression of these factors. The LBP significantly increased the survival rate of HaCaT cells and the SOD activity under UVB irradiation (P < 0.05), the LBP group at 50.0 mu g/mL showed the highest (P < 0.01) comparing with the control group, it could effectively reduce the mRNA and protein expression with a statistical significance. Therefore, the LBP (acidic polysaccharide, 22-25 KD) could be used for pharmaceutical and cosmetic candidate preventing UVB damage.