Effects of Lycium barbarum polysaccharides on expression of hypoxia inducible factor 1α and vascular endothelial growth factor in human immortalized keratinocytes cells irradiated by ultraviolet B

被引：0

作者：

Huang, Xiaowei ^{[1
]}

Liu, Yuexin ^{[1
]}

Ye, Hongbo ^{[1
]}

Lin, Zhe ^{[1
]}

Ma, Chao ^{[1
]}

Lin, He ^{[1
]}

Zhang, Hongchang ^{[2
]}

Chen, Hongyan ^{[3
]}

Ren, Jixiang ^{[3
]}

Yue, Hao ^{[2
]}

Lv, Guangfu ^{[2
]}

机构：

[1] Changchun Univ Chinese Med, Dept Sch Pharmaceut Sci, Changchun 130117, Peoples R China

[2] Changchun Univ Chinese Med, Dept Jinlin Ginseng Acad, Changchun 130117, Peoples R China

[3] Changchun Univ Chinese Med, Dept Affiliated Hosp, Changchun 130117, Peoples R China

来源：

MATERIALS EXPRESS | 2020年 / 10卷 / 12期

关键词：

Lycium barbarum Polysaccharides; Vascular Endothelial Growth Actor; Hypoxia Inducible Factor-1 alpha; UVB; HaCaT Cells; OXIDATIVE DAMAGE; PROSTATE-CANCER; SKIN-CANCER; UVA; DNA; APOPTOSIS; RADIATION; REPAIR;

D O I：

10.1166/mex.2020.1839

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Studies have shown that LBP (Lycium barbarum Polysaccharides) can protect the HaCaT (Human Immortalized keratinocytes cells) against the damage caused by UVB (Ultraviolet B). To probe the effects of LBP on the HIF-1 alpha and VEGF from HaCaT cells irradiated by UVB, HaCaT was disposed of LBP following by irradiating with different energy density of UVB for 24 h. The cell viability and activity of SOD (Super Oxide Dismutase) was measured by CCK-8 and enzyme-linked immunoassay, respectively. RT-PCR and western blot were employed to study the mRNA and protein expression of these factors. The LBP significantly increased the survival rate of HaCaT cells and the SOD activity under UVB irradiation (P < 0.05), the LBP group at 50.0 mu g/mL showed the highest (P < 0.01) comparing with the control group, it could effectively reduce the mRNA and protein expression with a statistical significance. Therefore, the LBP (acidic polysaccharide, 22-25 KD) could be used for pharmaceutical and cosmetic candidate preventing UVB damage.

引用

页码：2154 / 2159

页数：6

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