Preparation of Biodegradable Poly(Iactic-co-glycolic acid)/Polyethylene Glycol Copolymer and Its Application as a Carrier for Pigment Epithelium-Derived Factor

被引：4
作者：
Zhao, Junying [1 ]
Zhu, Yanji [1 ]
Wang, Yanyan [2 ]
Zhu, Jun [2 ]
Shen, Xi [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Shanghai 200025, Peoples R China
[2] Natl Engn Res Ctr Nanotechnol, Shanghai 200241, Peoples R China
来源：
CHINESE JOURNAL OF ORGANIC CHEMISTRY | 2017年 / 37卷 / 01期
基金：
中国国家自然科学基金;
关键词：
poly(lactic-co-glycolic acid)/polyethylene glycol (PLGA-PEG) molecule; coupling reaction; pigment epithelium-derived factor (PEDF); vascular endothelial growth factor (VEGF); ENDOTHELIAL GROWTH-FACTOR; CHOROIDAL NEOVASCULARIZATION; NANOPARTICLES; EXPRESSION; RELEASE; CELLS; PLGA; VEGF; PEG;
D O I：
10.6023/cjoc201608016
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A biodegradable poly(lactic-co-glycolic acid)/polyethylene glycol (PLGA-PEG) copolymer molecule was prepared by the simple and green coupling reaction between carboxyl group of poly(lactide-co-glycolide)-carboxylic acid endcap (PLGA-COOH) and amino group of poly(ethylene glycol)amine and carboxylic acid endcap (NH2-PEG-COOH) in the presence of l-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). The molecular structure of PLGA-PEG copolymer was characterized by H-1 NMR spectroscopy, and the grafting yield of polyethylene glycol (PEG) on poly(lactic-co-glycolic acid) (PLGA) was calculated. Furthermore, pigment epithelium-derived factor (PEDF) was encapsulated in the PLGA-PEG copolymer to prepare a PLGA-PEG/PEDF emulsion for highly efficient inhibition on vascular endothelial growth gene expression (VEGF). The results showed that the obtained PLGA-PEG copolymer was bio-safety and the PLGA-PEG/PEDF emulsion was a good agent for effective inhibition VEGF expression under different conditions. Thus, the PLGA-PEG copolymer can be used as a bio-carrier and the PLGA-PEG/PEDF emulsion can be used as a good angiogenesis inhibitor, which has a potential application for some diseases by anti angiogenesis.
引用
收藏
页码：203 / 208
页数：6
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