Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae)

被引:11
|
作者
Gonzalez-Gonzalez, Angelica [1 ,2 ]
Rubio-Melendez, Maria E. [3 ]
Ballesteros, Gabriel, I [1 ,2 ]
Ramirez, Claudio C. [1 ,2 ]
Palma-Millanao, Ruben [1 ,2 ]
机构
[1] Univ Talca, Ctr Mol & Funct Ecol, Talca, Chile
[2] Univ Talca, Inst Ciencias Biol, Talca, Chile
[3] Univ Talca, Fac Ingn, CBSM, Talca, Maule, Chile
来源
PEERJ | 2019年 / 7卷
关键词
RNAseq; Scarab beetle; Relative expression; Olfactory protein; Gustatory protein; Native pest; Transcriptome; ANTENNAL TRANSCRIPTOME; CORECEPTOR ORCO; LIGAND-BINDING; GENE FAMILIES; IDENTIFICATION; RECEPTORS; GENERATION; EVOLUTION; VOLATILE; OBP;
D O I
10.7717/peerj.7054
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this study, we addressed the sex- and tissue-specific expression patterns of odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) in Hylamorpha elegans (Burmeister), an important native scarab beetle pest species from Chile. Similar to other members of its family, this scarab beetle exhibit habits that make difficult to control the pest by conventional methods. Hence, alternative ways to manage the pest populations based on chemical communication and signaling (such as disrupting mating or host finding process) are highly desirable. However, developing pest-control methods based on chemical communication requires to understand the molecular basis for pheromone recognition/chemical perception in this species. Thus, with the aim of discovering olfaction-related genes, we obtained the first reference transcriptome assembly of H. elegans. We used different tissues of adult beetles from males and females: antennae and maxillary palps, which are well known for embedded sensory organs. Then, the expression of predicted odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) was analyzed by qRT-PCR. In total, 165 transcripts related to chemoperception were predicted. Of these, 16 OBPs, including one pheromone-binding protein (PBP), and four CSPs were successfully amplified by qRT-PCR. All of these genes were differentially expressed in the sensory tissues with respect to the tibial tissue that was used as a control. The single predicted PBP found was highly expressed in the antennal tissues, particularly in males, while several OBPs and one CSP showed male-biased expression patterns, suggesting that these proteins may participate in sexual recognition process. In addition, a single CSP was expressed at higher levels in female palps than in any other studied condition, suggesting that this CSP would participate in oviposition process. Finally, all four CSPs exhibited palp-biased expression while mixed results were obtained for the expression of the OBPs, which were more abundant in the palps than in the antennae. These results suggest that these chemoperception proteins would be interesting novel targets for control of H. elegans, thus providing a theoretical basis for further studies involving new pest control methods.
引用
收藏
页数:21
相关论文
共 12 条
  • [1] Odorant-Binding and Chemosensory Proteins in Anthonomus eugenii (Coleoptera: Curculionidae) and Their Tissue Expression
    Lechuga-Paredes, Pablo
    Segura-Leon, Obdulia Lourdes
    Cibrian-Tovar, Juan
    Torres-Huerta, Brenda
    Velazquez-Gonzalez, Julio Cesar
    Cruz-Jaramillo, Jose Luis
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (04)
  • [2] Potential Cooperations between Odorant-Binding Proteins of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)
    Wang, Bing
    Guan, Li
    Zhong, Tao
    Li, Kebin
    Yin, Jiao
    Cao, Yazhong
    PLOS ONE, 2013, 8 (12):
  • [3] Function and Immunocytochemical Localization of Two Novel Odorant-Binding Proteins in Olfactory Sensilla of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)
    Deng, Sisi
    Yin, Jiao
    Zhong, Tao
    Cao, Yazhong
    Li, Kebin
    CHEMICAL SENSES, 2012, 37 (02) : 141 - 150
  • [4] Sex- and Tissue-Specific Expression Profiles of Odorant Binding Protein and Chemosensory Protein Genes in &ITBradysia odoriphaga&IT (Diptera: Sciaridae)
    Zhao, Yunhe
    Ding, Jinfeng
    Zhang, Zhengqun
    Liu, Fang
    Zhou, Chenggang
    Mu, Wei
    FRONTIERS IN PHYSIOLOGY, 2018, 9
  • [5] Virtual Screening of Plant Volatile Compounds Reveals a High Affinity of Hylamorpha elegans (Coleoptera: Scarabaeidae) Odorant-Binding Proteins for Sesquiterpenes From Its Native Host
    Gonzalez-Gonzalez, Angelica, I
    Palma-Millanao, Ruben
    Yanez, Osvaldo
    Rojas, Maximiliano
    Mutis, Ana
    Venthur, Herbert
    Quiroz, Andres
    Ramirez, Claudio C.
    JOURNAL OF INSECT SCIENCE, 2016, 16
  • [6] Functional characterization of odorant-binding proteins from the scarab beetle Holotrichia oblita based on semiochemical-induced expression alteration and gene silencing
    Yin, Jiao
    Wang, Chaoqun
    Fang, Chiqin
    Zhang, Shuai
    Cao, Yazhong
    Li, Kebin
    Leal, Water S.
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2019, 104 : 11 - 19
  • [7] Antennal Transcriptome Evaluation and Analysis for Odorant-Binding Proteins, Chemosensory Proteins, and Suitable Reference Genes in the Leaf Beetle Pest Diorhabda rybakowi Weise (Coleoptera: Chrysomelidae)
    Xi, Bo-Xin
    Cui, Xiao-Ning
    Shang, Su-Qin
    Li, Guang-Wei
    Dewer, Youssef
    Li, Chang-Ning
    Hu, Gui-Xin
    Wang, Yan
    INSECTS, 2024, 15 (04)
  • [8] Expression Profiles and Functional Characterization of Two Odorant-Binding Proteins From the Apple Buprestid Beetle Agrilus mali (Coleoptera: Buprestidae)
    Cui, Xiaoning
    Liu, Deguang
    Sun, Keke
    He, Yang
    Shi, Xiaoqin
    JOURNAL OF ECONOMIC ENTOMOLOGY, 2018, 111 (03) : 1420 - 1432
  • [9] Odorant-Binding Proteins and Chemosensory Proteins in Spodoptera frugiperda: From Genome-Wide Identification and Developmental Stage-Related Expression Analysis to the Perception of Host Plant Odors, Sex Pheromones, and Insecticides
    Jia, Chen
    Mohamed, Amr
    Cattaneo, Alberto Maria
    Huang, Xiaohua
    Keyhani, Nemat O. O.
    Gu, Maiqun
    Zang, Liansheng
    Zhang, Wei
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (06)
  • [10] Tissue-specific transcriptomics, chromosomal localization, and phylogeny of chemosensory and odorant binding proteins from the red flour beetle Tribolium castaneum reveal subgroup specificities for olfaction or more general functions
    Dippel, Stefan
    Oberhofer, Georg
    Kahnt, Joerg
    Gerischer, Lizzy
    Opitz, Lennart
    Schachtner, Joachim
    Stanke, Mario
    Schuetz, Stefan
    Wimmer, Ernst A.
    Angeli, Sergio
    BMC GENOMICS, 2014, 15