Novel biopolimeric system for bone tissue engineering: crosslinked and plasticized chitosan/poly vinyl alcohol/hydroxiapatite scaffolds

被引：1

作者：

Pineda Castillo, Sergio Andres ^{[1
]}

Bernal Lopez, Cristian Camilo ^{[1
]}

Tovar Araujo, Favio Andres ^{[1
]}

Bernal-Ballen, Andres ^{[1
]}

Ramiro Segura-Puello, Hugo ^{[2
]}

Lorena Nieto-Mosquera, Diana ^{[2
]}

Milena Munoz-Forero, Diana ^{[2
]}

Viviana Guitarrero-Bustos, Sandra ^{[2
]}

Catalina Villamil-Ballesteros, Andrea ^{[2
]}

Munster, Lukas ^{[3
]}

机构：

[1] Univ Manuela Beltran, Grp Invest Ingn Biomed Vicerrectoria Invest, Ave Circunvalar 60-00, Bogota, Colombia

[2] Univ Manuela Beltran, Lab Invest Salud, Ave Circunvalar 60-00, Bogota, Colombia

[3] Tomas Bata Univ Zlin, Univ Inst, Ctr Polymer Syst, Trida Tomase Bati 5678, Zlin 76001, Czech Republic

来源：

2018 IX INTERNATIONAL SEMINAR OF BIOMEDICAL ENGINEERING (SIB) | 2018年

关键词：

chitosan; polyvinyl alcohol; hydroxiapatite; tissue engineering; bone regeneration; HYDROXYAPATITE;

D O I：

10.1109/SIB.2018.8467728

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Porous scaffolds made of chitosan, polyvinyl alcohol and hydroxyapatite were prepared via freeze-drying method. In this matter, three different volume ratios of polymer solutions (1:1, 1:3, 3:1) and constant hydroxyapatite mass ratio were blended. Glutaraldehyde and glycerol were added as a polymer-chain crosslinker and plasticizer, respectively. The obtained scaffolds were used to test bone proliferation and potential for bone regeneration and they were characterized through FTIR, mechanical tests, cell cultures and swelling tests. Every quantitative measurement was statistically tested using two-way analysis of variance (ANOVA) with rho < 0.05. Partial results from cell culture have been obtained, showing faster cell differentiation in the scaffolds combined with glutaraldehyde and glycerol. Crosslinked scaffolds showed better swelling conditions than those that were not subject to this chemical reaction. Besides, samples with higher chitosan ratio exhibited better swelling behavior. Experiments suggest that the bio-polymeric system has potential for bone tissue regeneration.

引用

页数：7

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