The Structure of the Catalytic Domain of a Plant Cellulose Synthase and Its Assembly into Dimers

被引:50
作者
Olek, Anna T. [1 ]
Rayon, Catherine [1 ]
Makowski, Lee [2 ,3 ]
Kim, Hyung Rae [4 ]
Ciesielski, Peter [5 ]
Badger, John [6 ]
Paul, Lake N. [7 ]
Ghosh, Subhangi [4 ]
Kihara, Daisuke [4 ,8 ]
Crowley, Michael [5 ]
Himmel, Michael E. [5 ]
Bolin, Jeffrey T. [4 ]
Carpita, Nicholas C. [1 ,4 ,7 ]
机构
[1] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA
[2] Northeastern Univ, Dept Bioengn, Boston, MA 02115 USA
[3] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA
[4] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[5] Natl Renewable Energy Lab, Biomol Sci Grp, Golden, CO 80401 USA
[6] DeltaG Technol, San Diego, CA 92122 USA
[7] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA
[8] Purdue Univ, Dept Comp Sci, W Lafayette, IN 47907 USA
基金
新加坡国家研究基金会; 美国国家科学基金会; 美国国家卫生研究院;
关键词
SMALL-ANGLE SCATTERING; X-RAY-SCATTERING; PROTEIN-STRUCTURE PREDICTION; SYNTHESIZING COMPLEX; FOLD-RECOGNITION; BINDING; BIOSYNTHESIS; SERVER; GENE; MECHANISMS;
D O I
10.1105/tpc.114.126862
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cellulose microfibrils are para-crystalline arrays of several dozen linear (1 -> 4)-beta-D-glucan chains synthesized at the surface of the cell membrane by large, multimeric complexes of synthase proteins. Recombinant catalytic domains of rice (Oryza sativa) CesA8 cellulose synthase form dimers reversibly as the fundamental scaffold units of architecture in the synthase complex. Specificity of binding to UDP and UDP-Glc indicates a properly folded protein, and binding kinetics indicate that each monomer independently synthesizes single glucan chains of cellulose, i.e., two chains per dimer pair. In contrast to structure modeling predictions, solution x-ray scattering studies demonstrate that the monomer is a two-domain, elongated structure, with the smaller domain coupling two monomers into a dimer. The catalytic core of the monomer is accommodated only near its center, with the plant-specific sequences occupying the small domain and an extension distal to the catalytic domain. This configuration is in stark contrast to the domain organization obtained in predicted structures of plant CesA. The arrangement of the catalytic domain within the CesA monomer and dimer provides a foundation for constructing structural models of the synthase complex and defining the relationship between the rosette structure and the cellulose microfibrils they synthesize.
引用
收藏
页码:2996 / 3009
页数:14
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