A rapid one-step multiplex RT-PCR assay for the simultaneous detection of five citrus viroids in China

被引：33

作者：

Wang, Xuefeng ^{[1
]}

Zhou, Changyong ^{[1
]}

Tang, Kezhi ^{[1
]}

Zhou, Yan ^{[1
]}

Li, Zhongan ^{[1
]}

机构：

[1] Southwest Univ, Natl Citrus Virus Exclus Ctr, Citrus Res Inst, Chongqing 400712, Peoples R China

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2009年 / 124卷 / 01期

关键词：

Citrus viroids; Multiplex; One-step RT-PCR; Nucleic acid extraction method; NUCLEOTIDE-SEQUENCE; SECONDARY STRUCTURE; EXOCORTIS; GRAPEFRUIT; VIRUS;

D O I：

10.1007/s10658-008-9386-y

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Citrus plants are natural hosts of five viroid species and large numbers of sequence variants. In this paper a simple and sensitive one step multiplex RT-PCR protocol with an internal control was utilised to simultaneously detect and differentiate five citrus viroids: Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd), Hop stunt viroid (HSVd), Citrus viroid-III (CVd-III) and Citrus viroid-IV (CVd-IV). In addition, a micro and rapid total nucleic acid extraction method was developed and the protocol applied to evaluate the occurrence and distribution of citrus viroids in China.

引用

页码：175 / 180

页数：6

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