ABI3 controls embryo degreening through Mendel's I locus

被引:147
作者
Delmas, Frederic [1 ,2 ,3 ]
Sankaranarayanan, Subramanian [4 ]
Deb, Srijani [4 ]
Widdup, Ellen [4 ]
Bournonville, Celine [2 ,3 ]
Bollier, Norbert [2 ,3 ]
Northey, Julian G. B. [1 ]
McCourt, Peter [1 ]
Samuel, Marcus A. [4 ]
机构
[1] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON M5S 3B2, Canada
[2] Univ Bordeaux, Unite Mixte Rech Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
[3] INRA, Unite Mixte Rech Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
[4] Univ Calgary, Dept Biol Sci, Calgary, AB T2N 1N4, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
freezing tolerance; nondormant; CHLOROPHYLL CATABOLITE REDUCTASE; ABSCISIC-ACID; ARABIDOPSIS-THALIANA; FREEZING TOLERANCE; LEAF SENESCENCE; GREEN-SEED; STAY-GREEN; PROTEIN; EXPRESSION; PHEOPHORBIDE;
D O I
10.1073/pnas.1308114110
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Chlorophyll (chl) is essential for light capture and is the starting point that provides the energy for photosynthesis and thus plant growth. Obviously, for this reason, retention of the green chlorophyll pigment is considered a desirable crop trait. However, the presence of chlorophyll in mature seeds can be an undesirable trait that can affect seed maturation, seed oil quality, and meal quality. Occurrence of mature green seeds in oil crops such as canola and soybean due to unfavorable weather conditions during seed maturity is known to cause severe losses in revenue. One recently identified candidate that controls the chlorophyll degradation machinery is the stay-green gene, SGR1 that was mapped to Mendel's I locus responsible for cotyledon color (yellow versus green) in peas. A defect in SGR1 leads to leaf stay-green phenotypes in Arabidopsis and rice, but the role of SGR1 in seed degreening and the signaling machinery that converges on SGR1 have remained elusive. To decipher the gene regulatory network that controls degreening in Arabidopsis, we have used an embryo stay-green mutant to demonstrate that embryo degreening is achieved by the SGR family and that this whole process is regulated by the phytohormone abscisic acid (ABA) through ABSCISIC ACID INSENSITIVE 3 (ABI3); a B3 domain transcription factor that has a highly conserved and essential role in seed maturation, conferring desiccation tolerance. Misexpression of ABI3 was sufficient to rescue cold-induced green seed phenotype in Arabidopsis. This finding reveals a mechanistic role for ABI3 during seed degreening and thus targeting of this pathway could provide a solution to the green seed problem in various oil-seed crops.
引用
收藏
页码:E3888 / E3894
页数:7
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