Three auditory brainstem response (ABR) methods tested and compared in two anuran species

被引:8
作者
Lauridsen, Tanya B. [1 ]
Brandt, Christian [2 ]
Christensen-Dalsgaard, Jakob [1 ]
机构
[1] Univ Southern Denmark, Dept Biol, DK-5230 Odense, Denmark
[2] Univ Southern Denmark, Dept Clin Res, DK-5230 Odense, Denmark
关键词
ABR; Auditory brainstem response; Amphibian; Hearing; Hearing sensitivity; FREQUENCY DISCRIMINATION; EVOKED POTENTIALS; PHASE-LOCKING; NERVE-FIBERS; HEARING; SENSITIVITY; THRESHOLD;
D O I
10.1242/jeb.237313
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hearing sensitivity has been extensively investigated, often by measuring the auditory brainstem response (ABR). ABR measurements are relatively non-invasive, easy to reproduce, and allow the assessment of sensitivity when psychophysical data are difficult to obtain. However, the experimental methods differ greatly in respect to stimulation, which may result in different audiograms. We used three different methods in the same individual frogs: stimulating with brief tone bursts (tABR), long-duration tones (ItABR) and masked ABR (mABR), where transients are masked by a long-duration sinusoid, and the sensitivity is assessed by the difference between unmasked and masked ABR. We measured sensitivity in a range from 100 to 3500 Hz, and the resulting audiograms show two sensitivity peaks at 400-600 Hz and 1500-1600 Hz (both sensitive down to 30 dB re. 20 mu Pa). We found similar results below 1000 Hz, but when stimulating with long-duration tones, the sensitivity decreased more rapidly above this frequency. We showed that the frequency specificity of tone bursts becomes poorly defined with shorter duration at low frequencies. Comparisons between subjectively (visual inspection by researchers) and objectively (thresholds defined by signal-to-noise ratio) defined audiograms showed very little variation. In conclusion, the mABR method gave the most sensitive audiograms. The tABR method showed a similar audiogram when using relatively long-duration tone bursts (25 ms). The ItABR method is not a good choice for studying hearing thresholds above 1000 Hz because of the bias introduced by spike rate saturation in the nerve fibers and their inability to phase lock.
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页数:8
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共 34 条
  • [1] The paradox of hearing at the lek: auditory sensitivity increases after breeding in female gray treefrogs (Hyla chrysoscelis)
    Baugh, Alexander T.
    Bee, Mark A.
    Gall, Megan D.
    [J]. JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 2019, 205 (04): : 629 - 639
  • [2] DERIVED GUINEA-PIG COMPOUND 8TH-NERVE ACTION-POTENTIALS TO CONTINUOUS PURE-TONES
    BERLIN, CI
    HOOD, LJ
    BARLOW, EK
    MOREHOUSE, CR
    SMITH, EG
    [J]. HEARING RESEARCH, 1991, 52 (02) : 271 - 280
  • [3] Brandt C., 2007, 1 INT S AUD AUD RES, P241
  • [4] The Masked ABR (mABR): a New Measurement Method for the Auditory Brainstem Response
    Brandt, Christian
    Brande-Lavridsen, Nanna
    Christensen-Dalsgaard, Jakob
    [J]. JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY, 2018, 19 (06): : 753 - 761
  • [5] Auditory brainstem responses in adult budgerigars (Melopsittacus undulatus)
    Brittan-Powell, EF
    Dooling, RJ
    Gleich, O
    [J]. JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA, 2002, 112 (03) : 999 - 1008
  • [6] The auditory brainstem response in two lizard species
    Brittan-Powell, Elizabeth F.
    Christensen-Dalsgaard, Jakob
    Tang, Yezhong
    Carr, Catherine
    Dooling, Robert J.
    [J]. JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA, 2010, 128 (02) : 787 - 794
  • [7] ACOUSTIC COMMUNICATION IN GRASS FROG (RANA-T-TEMPORARIA L) - CALLS, AUDITORY-THRESHOLDS AND BEHAVIORAL-RESPONSES
    BRZOSKA, J
    WALKOWIAK, W
    SCHNEIDER, H
    [J]. JOURNAL OF COMPARATIVE PHYSIOLOGY, 1977, 118 (02): : 173 - 186
  • [8] Assessing stimulus and subject influences on auditory evoked potentials and their relation to peripheral physiology in green treefrogs (Hyla cinerea)
    Buerkle, Nathan P.
    Schrode, Katrina M.
    Bee, Mark A.
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2014, 178 : 68 - 81
  • [9] Burkard R.F., 2007, AUDITORY EVOKED POTE, P229
  • [10] Better than fish on land? Hearing across metamorphosis in salamanders
    Christensen, Christian Bech
    Lauridsen, Henrik
    Christensen-Dalsgaard, Jakob
    Pedersen, Michael
    Madsen, Peter Teglberg
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2015, 282 (1802)