Stimulation of excitatory amino acid release from adult mouse brain glia subcellular particles by high mobility group box 1 protein

被引:67
作者
Pedrazzi, Marco
Raiteri, Luca
Bonanno, Giambattista
Patrone, Mauro
Ledda, Sabina
Passalacqua, Mario
Milanese, Marco
Melloni, Edon
Raiteri, Maurizio
Pontremoli, Sandro
Sparatore, Bianca
机构
[1] Univ Genoa, Dept Expt Med, Pharmacol & Toxicol Sect, I-16148 Genoa, Italy
[2] Univ Genoa, Ctr Excellence Biomed Res, Genoa, Italy
[3] Univ Eastern Piedmont Amedeo Avogadro, Dept Environm & Life Sci, Alessandria, Italy
关键词
glial glutamate-aspartate transporter; gliosomes; glutamate release; high mobility group box 1; receptor for advanced glycation end products; synaptosomes;
D O I
10.1111/j.1471-4159.2006.04120.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The multifunctional protein high mobility group box 1 (HMGB1) is expressed in hippocampus and cerebellum of adult mouse brain. Our aim was to determine whether HMGB1 affects glutamatergic transmission by monitoring neurotransmitter release from glial (gliosomes) and neuronal (synaptosomes) re-sealed subcellular particles isolated from cerebellum and hippocampus. HMGB1 induced release of the glutamate analogue [H-3]D-aspartate form gliosomes in a concentration-dependent manner, whereas nerve terminals were insensitive to the protein. The HMGB1-evoked release of [H-3]D-aspartate was independent of modifications of cytosolic Ca2+, but it was blocked by DL-threo-beta-benzyloxyaspartate (DL-TBOA), an inhibitor of glutamate transporters. HMGB1 also stimulated the release of endogenous glutamate in a Ca2+-independent and DL-TBOA-sensitive manner. These findings suggest the involvement of carrier-mediated release. Moreover, dihydrokainic acid, a selective inhibitor of glutamate transporter 1 (GLT1), does not block the effect of HMGB1, indicating a role for the glial glutamate-aspartate transporter (GLAST) subtype in this response. We also demonstrate that HMGB1/glial particles association is promoted by Ca2+. Furthermore, although HMGB1 can physically interact with GLAST and the receptor for advanced glycation end products (RAGE), only its binding with RAGE is promoted by Ca2+. These results suggest that the HMGB1 cytokine could act as a modulator of glutamate homeostasis in adult mammal brain.
引用
收藏
页码:827 / 838
页数:12
相关论文
共 50 条
[21]   CARRIER-MEDIATED RELEASE OF NEUROTRANSMITTERS [J].
LEVI, G ;
RAITERI, M .
TRENDS IN NEUROSCIENCES, 1993, 16 (10) :415-419
[22]   Differential control of synaptic and ectopic vesicular release of glutamate [J].
Matsui, K ;
Jahr, CE .
JOURNAL OF NEUROSCIENCE, 2004, 24 (41) :8932-8939
[23]   High mobility group box protein 1: An endogenous signal for dendritic cell maturation and Th1 polarization [J].
Messmer, D ;
Yang, H ;
Telusma, G ;
Knoll, F ;
Li, JH ;
Messmer, B ;
Tracey, KJ ;
Chiorazzi, N .
JOURNAL OF IMMUNOLOGY, 2004, 173 (01) :307-313
[24]   High affinity binding and overlapping localization of neurocan and phosphacan protein-tyrosine phosphatase-ζ/β with tenascin-R, amphoterin, and the heparin-binding growth-associated molecule [J].
Milev, P ;
Chiba, A ;
Häring, M ;
Rauvala, H ;
Schachner, M ;
Ranscht, B ;
Margolis, RK ;
Margolis, RU .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (12) :6998-7005
[25]   Regulated expression and subcellular localization of HMGB1, a chromatin protein with a cytokine function [J].
Müller, S ;
Ronfani, L ;
Bianchi, ME .
JOURNAL OF INTERNAL MEDICINE, 2004, 255 (03) :332-343
[26]  
NAITO S, 1983, J BIOL CHEM, V258, P696
[27]   UPTAKE OF TRANSMITTER AMINO-ACIDS BY GLIAL PLASMALEMMAL VESICLES FROM DIFFERENT REGIONS OF RAT CENTRAL-NERVOUS-SYSTEM [J].
NAKAMURA, Y ;
KUBO, H ;
KATAOKA, K .
NEUROCHEMICAL RESEARCH, 1994, 19 (09) :1145-1150
[28]   GLIAL PLASMALEMMAL VESICLES - A SUBCELLULAR FRACTION FROM RAT HIPPOCAMPAL HOMOGENATE DISTINCT FROM SYNAPTOSOMES [J].
NAKAMURA, Y ;
IGA, K ;
SHIBATA, T ;
SHUDO, M ;
KATAOKA, K .
GLIA, 1993, 9 (01) :48-56
[30]   Further characterization of high mobility group box 1 (HMGB1) as a proinflammatory cytokine: central nervous system effects [J].
O'Connor, KA ;
Hansen, MK ;
Pugh, CR ;
Deak, MM ;
Biedenkapp, JC ;
Milligan, ED ;
Johnson, JD ;
Wang, HC ;
Maier, SF ;
Tracey, KJ ;
Watkins, LR .
CYTOKINE, 2003, 24 (06) :254-265