An amperometric urea biosensor based on covalently immobilized urease on an electrode made of hyperbranched polyester functionalized gold nanoparticles

被引：95

作者：

Tiwari, Ashutosh ^{[1
]}

Aryal, Santosh ^{[1
]}

Pilla, Srikanth ^{[1
]}

Gong, Shaoqin ^{[1
,2
]}

机构：

[1] Univ Wisconsin, Dept Mech Engn, Milwaukee, WI 53211 USA

[2] Univ Wisconsin, Dept Mat, Milwaukee, WI 53211 USA

来源：

TALANTA | 2009年 / 78卷 / 4-5期

基金：

美国国家科学基金会;

关键词：

Urea biosensor; Amperometric detection; Covalent immobilization; Boltron (R) H40; Gold nanoparticles; ENZYME-ELECTRODE; ELECTROCHEMICAL DETECTION; BLOOD-SAMPLES;

D O I：

10.1016/j.talanta.2009.02.038

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric biosensor was fabricated for the quantitative determination of urea in aqueous medium using hematein, a pH-sensitive natural dye. The urease(Urs) was covalently immobilized onto an electrode made of gold nanoparticles functionalized with hyperbranched polyester-Boltron (R) H40 (H40-Au) coated onto an indium-tin oxide (ITO) covered glass substrate. The covalent linkage between the Urs enzyme and H40-Au nanoparticles provided the resulting enzyme electrode (Urs/H40-Au/ITO) with a high level of enzyme immobilization and excellent lifetime stability. The response studies were carried out as a function of urea concentration with amperometric and photometric measurements. The biosensor based on Urs/H40-Au/ITO as the working electrode showed a linear current response to the urea concentration ranging from 0.01 to 35 mM. The urea biosensor exhibited a sensitivity of 7.48 nA/mM with a response time of 3s. The Michaelis-Menten constant for the Urs/H40-Au/ITO biosensor was calculated to be 0.96 mM, indicating the Urs enzyme immobilized on the electrode surface had a high affinity to Urea. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1401 / 1407

页数：7

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