In vitro and in vivo imaging application of a 1,8-naphthalimide-derived Zn2+ fluorescent sensor with nuclear envelope penetrability

被引：68

作者：

Zhang, Changli ^{[1
,2
]}

Liu, Zhipeng ^{[3
]}

Li, Yunling ^{[1
]}

He, Weijiang ^{[1
]}

Gao, Xiang ^{[4
]}

Guo, Zijian ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Inst Coordinat Chem, State Key Lab Coordinat Chem, Nanjing 210093, Jiangsu, Peoples R China

[2] Nanjing Xiaozhuang Coll, Dept Chem, Nanjing 210017, Jiangsu, Peoples R China

[3] Liaocheng Univ, Dept Chem, Liaocheng 252059, Peoples R China

[4] Nanjing Univ, Anim Model Res Ctr, Nanjing 210061, Jiangsu, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 97期

基金：

中国国家自然科学基金;

关键词：

ZINC; METALLOTHIONEIN; FLUOROPHORE; PROBE;

D O I：

10.1039/c3cc46862c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A newly developed fluorescent sensor, Naph-BPEA, shows a specific turn-on response to Zn2+ and can be excited by visible light. The in situ nuclear Zn2+ imaging in HeLa and HepG2 cells reveals the nuclear envelope penetrability of the sensor. The specific sensor location in a zebrafish larva was also demonstrated.

引用

页码：11430 / 11432

页数：3

共 15 条

[1] Counting the zinc-proteins encoded in the human genome [J].

Andreini, C ;

Banci, L ;

Bertini, I ;

Rosato, A .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (01) :196-201

[2] Zinc through the three domains of life [J].

Andreini, Claudia ;

Banci, Lucia ;

Bertini, Ivano ;

Rosato, Antonio .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (11) :3173-3178

[3] Recent advances in the development of 1,8-naphthalimide based DNA targeting binders, anticancer and fluorescent cellular imaging agents [J].

Banerjee, Swagata ;

Veale, Emma B. ;

Phelan, Caroline M. ;

Murphy, Samantha A. ;

Tocci, Gillian M. ;

Gillespie, Lisa J. ;

Frimannsson, Daniel O. ;

Kelly, John M. ;

Gunnlaugsson, Thorfinnur .

CHEMICAL SOCIETY REVIEWS, 2013, 42 (04) :1601-1618

[4]

Canzoniero LMT, 1999, J NEUROSCI, V19

[5] MEASUREMENT OF ZINC IN HEPATOCYTES BY USING A FLUORESCENT-PROBE, ZINQUIN - RELATIONSHIP TO METALLOTHIONEIN AND INTRACELLULAR ZINC [J].

COYLE, P ;

ZALEWSKI, PD ;

PHILCOX, JC ;

FORBES, IJ ;

WARD, AD ;

LINCOLN, SF ;

MAHADEVAN, I ;

ROFE, AM .

BIOCHEMICAL JOURNAL, 1994, 303 :781-786

[6] Intracellular zinc distribution and transport in C6 rat glioma cells [J].

Haase, H ;

Beyersmann, D .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 296 (04) :923-928

[7] An NBD fluorophore-based sensitive and selective fluorescent probe for zinc ion [J].

Jiang, Wei ;

Fu, Qingquan ;

Fan, Hongyou ;

Wang, Wei .

CHEMICAL COMMUNICATIONS, 2008, (02) :259-261

[8] New Alternately Colored FRET Sensors for Simultaneous Monitoring of Zn2+ in Multiple Cellular Locations [J].

Miranda, Jose G. ;

Weaver, Amanda L. ;

Qin, Yan ;

Park, J. Genevieve ;

Stoddard, Caitlin I. ;

Lin, Michael Z. ;

Palmer, Amy E. .

PLOS ONE, 2012, 7 (11)

[9] Visible Light Excitable Zn2+ Fluorescent Sensor Derived from an Intramolecular Charge Transfer Fluorophore and Its in Vitro and in Vivo Application [J].

Qian, Fang ;

Zhang, Changli ;

Zhang, Yumin ;

He, Weijiang ;

Gao, Xiang ;

Hu, Ping ;

Guo, Zijian .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (04) :1460-1468

[10] Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors [J].

Qin, Yan ;

Dittmer, Philip J. ;

Park, J. Genevieve ;

Jansen, Katarina B. ;

Palmer, Amy E. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (18) :7351-7356

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