Influence of Protein Size on Surface-Enhanced Raman Scattering (SERS) Spectra in Binary Protein Mixtures

被引：9

作者：

Avci, Ertug ^{[1
]}

Culha, Mustafa ^{[1
]}

机构：

[1] Yeditepe Univ, Dept Genet & Bioengn, TR-34755 Istanbul, Turkey

来源：

APPLIED SPECTROSCOPY | 2014年 / 68卷 / 08期

关键词：

SERS; Protein; Binary mixture; Label-free; Detection; Principal component analysis; LABEL-FREE DETECTION; PLASMONIC NANOPARTICLES; GOLD NANOPARTICLES; SILVER COLLOIDS; SPECTROSCOPY; ORIENTATION; FIBRINOGEN; DIFFERENCE; MONOLAYERS; STABILITY;

D O I：

10.1366/13-07445

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The size-dependent interactions of eight blood proteins with silver nanoparticles (AgNPs) in their binary mixtures were investigated using surface-enhanced Raman scattering (SERS). Principal component analysis (PCA) was performed on the SERS spectra of each binary mixture, and the differentiation ability of the mixtures was tested. It was found that the effect of relative concentration change on the SERS spectra of the binary mixtures of small proteins could be detected using PCA. However, this change was not observed with the binary mixtures of large proteins. This study demonstrated that the relative interactions of the smaller proteins with an average size of 50 nm AgNPs smaller than the large proteins could be monitored, and this information can be used for the detection of proteins in protein mixtures.

引用

页码：890 / 899

页数：10

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