Characterization and biological functions of sulfated polysaccharides from sulfated-salt treatment of Antrodia cinnamomea

被引:44
作者
Cheng, Jing-Jy [1 ]
Huang, Nai-Luei [1 ]
Lur, Huu-Sheng [2 ]
Kuo, Chung-Io [2 ]
Lu, Mei-Kuang [1 ]
机构
[1] Natl Res Inst Chinese Med, Taipei 112, Taiwan
[2] Natl Taiwan Univ, Dept Agron, Taipei, Taiwan
关键词
Antrodia cinnamomea; Sulfated polysaccharides; Angiogenesis; Pheochromocytoma cells; Neuroprotection; Apoptosis; CAMPHORATA MYCELIA; TUMOR-GROWTH; ANGIOGENESIS; TRITERPENOIDS; GLYCANS; CELLS; INVOLVEMENT; INHIBITION; APOPTOSIS; ISOFORM;
D O I
10.1016/j.procbio.2008.12.012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sulfated polysaccharides (SPSs) of Antrodia cinnamomea were extracted by an exhaustive papain digestion and characterized, and their biological functions were evaluated. In this study, we demonstrated the existence of SPSs in the medicinal fungus. A. cinnamomea. Since no SPSs had previously been identified in any fungal organism, we attempted to characterize those from A. cinnamomea. SPSs from A. cinnamomea inhibited in vitro Matrigel tube formation, in an angiogenesis model, in a dose-dependent manner. Furthermore, using serum deprivation-induced apoptosis in neuronal-like PC12 cells as a stress model, the SPSs of A. cinnamomea were effective in preventing serum-deprived apoptosis. Compositional analysis revealed that myo-inositol, fucose, galactose, and glucose were the neutral sugars in SPSs of A. cinnamomea, and these SPSs had a high sulfate content. The sulfation degree paralleled their antiangiogenic and neuroprotective activities. In this work, we report novel data on the structure, antiangiogenic, and neuroprotective effects of these fungal SPSs. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:453 / 459
页数:7
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