Snail modulates the assembly of fibronectin via α5 integrin for myocardial migration in zebrafish embryos

被引：19
作者：
Qiao, Liangjun [1 ]
Gao, Hongwei [1 ]
Zhang, Ting [1 ]
Jing, Lulu [1 ]
Xiao, Chun [1 ]
Xiao, Yue [1 ]
Luo, Ning [1 ]
Zhu, Hongyan [1 ]
Meng, Wentong [1 ]
Xu, Hong [1 ]
Mo, Xianming [1 ]
机构：
[1] Sichuan Univ, West China Hosp, Ctr Med Stem Cell Biol, Lab Stem Cell Biol,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
来源：
SCIENTIFIC REPORTS | 2014年 / 4卷
关键词：
IN-SITU HYBRIDIZATION; NEURAL CREST; WILD-TYPE; EXPRESSION; CUSHION; GENE; ORGANIZATION; SPADETAIL; MESODERM; FAMILY;
D O I：
10.1038/srep04470
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The Snail family member snail encodes a zinc finger-containing transcriptional factor that is involved in heart formation. Yet, little is known about how Snail regulates heart development. Here, we identified that one of the duplicated snail genes, snai1b, was expressed in the heart region of zebrafish embryos. Depletion of Snai1b function dramatically reduced expression of alpha 5 integrin, disrupted Fibronectin layer in the heart region, especially at the midline, and prevented migration of cardiac precursors, resulting in defects in cardiac morphology and function in zebrafish embryos. Injection of alpha 5 beta 1 protein rescued the Fibronectin layer and then the myocardial precursor migration in snai1b knockdown embryos. The results provide the molecular mechanism how Snail controls the morphogenesis of heart during embryonic development.
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页数：6
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