Electrochemical labeled DNA probe for the detection of sequence-specific DNA

被引：47

作者：

Xu, C ^{[1
]}

He, PG ^{[1
]}

Fang, YZ ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 411卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

electrochemical labeled probe; DNA; hybridization;

D O I：

10.1016/S0003-2670(00)00728-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical labeled DNA probe for the detection of sequence-specific DNA is discussed. The denatured calf-thymus DNA was successfully labeled with the electrochemical active reagent ferrocenecarboxaldehyde (FCA) by 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC). This FCA labeled DNA probe is used to hybridize with the sample DNA sequence accumulated on the surface of a graphite electrode. Only the complementary sequence (cDNA) can form a double-stranded DNA (dsDNA) with the FCA labeled DNA probe. The anodic waves of the FCA bound to the double-stranded DNA (dsDNA) by differential pulse voltammetry (DPV) are used for the detection of cDNA. The anodic peak currents (i(pa)) Of FCA are related to the concentration of cDNA sequence between 1.0x10(-8) and 5.0x10(-6) mol l(-1). The detection limit is 5x10(-9) mol l(-1)(S/N=3). The probe showed high sensitivity and selectivity. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：31 / 36

页数：6

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