Multidimensional HPLC mapping method for the structural analysis of anionic N-glycans

被引：8
作者：
Yagi, Hirokazu [1 ,2 ]
Kato, Koichi [1 ,3 ,4 ,5 ,6 ]
机构：
[1] Nagoya City Univ, Grad Sch Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan
[2] Natl Inst Nat Sci, Natl Inst Physiol Sci, Okazaki, Aichi 4448787, Japan
[3] Natl Inst Nat Sci, Inst Mol Sci, Okazaki, Aichi 4448787, Japan
[4] Natl Inst Nat Sci, Okazaki Inst Integrat Biosci, Okazaki, Aichi 4448787, Japan
[5] GLYENCE Co Ltd, Chikusa Ku, Nagoya, Aichi 4640858, Japan
[6] Ochanomizu Univ, Glycosci Inst, Bunkyo Ku, Tokyo 1128610, Japan
来源：
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY | 2009年 / 21卷 / 118期
基金：
日本学术振兴会;
关键词：
HPLC mapping method; sulfated oligosaccharide; glucuronylated oligosaccharide; N-glycosylation profile; glycomics; CAENORHABDITIS-ELEGANS; LINKED GLYCANS; OLIGOSACCHARIDES; EXPRESSION; GLYCOSYLATION; HEMAGGLUTININ; PATTERN; PROTEIN; EMBRYO;
D O I：
10.4052/tigg.21.95
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The multidimensional HPLC mapping method developed by Dr. Noriko Takahashi and coworkers has been widely recognized as a useful tool for the structural analysis of neutral and sialyl N-glycans. The utility of this method would be further extended if it could be used to analyze anionic oligosaccharides, including sulfated or glucuronylated glycans, since they play important roles in cell-cell communications. Hence, we developed HPLC maps of sulfated and glucuronylated oligosaccharides produced by recombinant sulfotransferases and glycosyltransferases. Here, we describe the HPLC map of anionic glycans and demonstrate its utility by applying this map for the characterization of sulfotransferase N-acetylglucosamine 6-O-sulfotransferase-1 (GlcNAc6ST-1) and glycosylation profiling of influenza virus.
引用
收藏
页码：95 / 104
页数：10
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