Ex vivo synthesized cytokines as a biomarker of stroke-associated pneumonia

被引:2
作者
Gradek-Kwinta, Elzbieta [1 ]
Czyzycki, Mateusz [1 ]
Weglarczyk, Kazimierz [2 ]
Slowik, Agnieszka [1 ]
Siedlar, Maciej [2 ]
Dziedzic, Tomasz [1 ]
机构
[1] Jagiellonian Univ Med Coll, Dept Neurol, Ul Bot 3, PL-31503 Krakow, Poland
[2] Jagiellonian Univ Med Coll, Inst Paediat, Dept Clin Immunol, Krakow, Poland
关键词
Pneumonia; Stroke; Cytokines; Biomarker; ACUTE ISCHEMIC-STROKE; BLOOD STIMULATION; WHOLE-BLOOD; MORTALITY; INFECTION; ENDOTOXIN; RELEASE; IL-6;
D O I
10.1016/j.cca.2020.07.035
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objectives: We aimed to determine a profile of ex vivo released cytokines in patients with stroke-associated pneumonia (SAP) and to assess the clinical utility of individual cytokines and their combination as a biomarker of SAP. Methods: We included 279 ischemic stroke patients (median age: 69 years; 41.6% women). We collected blood samples at day 3 after the onset of stroke and stimulated them ex vivo with lipopolysaccharide (LPS). We measured the LPS-induced cytokine concentrations (TNF alpha, IP-10, IL-1 beta, IL-6, IL-8, IL-10 and IL-12p70) as well as a plasma IL-6 level as a marker of systemic inflammation. We assessed the discriminatory ability of cytokines by calculating the area under the receiver operating characteristic curve (AUC). Results: During first 5 days after stroke pneumonia occurred in 7.2% of patients. Patients with SAP had lower ex vivo release of TNF alpha, IL-1 beta, IL-12, IP-10 and a higher level of circulating IL-6 than patients without SAP. The multimarker score composed of ex vivo synthesized IL-12, IP-10, and plasma IL-6 had better discriminatory properties than individual cytokines (AUC: 0.90). Conclusions: Our results suggest the potential utility of ex vivo synthesized cytokines as a biomarker of SAP.
引用
收藏
页码:260 / 263
页数:4
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