The aim of this work was to employ optimization strategy based on statistical experimental designs to enhance the polysaccharides extraction yield from Gynostemma pentaphyllum. The effects of three independent variables on the polysaccharides extraction yield were investigated and the optimal conditions were evaluated by means of Box-Behnken design. The optimal conditions are as follows: ratio of water to raw material 31, extraction temperature 90 degrees C and extraction time 2.5h. Under these conditions, the polysaccharides yield is 3.80 +/- 0.15%(N=5), which is agreed closely with the predicted yield value.
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Box G.E.P., 1960, Technometrics, V2, P455, DOI 10.1080/00401706.1960.10489912
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Lv, You
Yang, Xingbin
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Yang, Xingbin
Zhao, Yan
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Fourth Mil Med Univ, Fac Pharmaceut Sci, Xian 710032, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Zhao, Yan
Ruan, Yun
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Ruan, Yun
Yang, Ying
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Yang, Ying
Wang, Zhezhi
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
机构:
Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Lv, You
Yang, Xingbin
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Yang, Xingbin
Zhao, Yan
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机构:
Fourth Mil Med Univ, Fac Pharmaceut Sci, Xian 710032, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Zhao, Yan
Ruan, Yun
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Ruan, Yun
Yang, Ying
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China
Yang, Ying
Wang, Zhezhi
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Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R ChinaShaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China