Induced DNA recombination by Cre recombinase protein transduction

被引：40

作者：

Joshi, SK

Hashimoto, K

Koni, PA

机构：

[1] Med Coll Georgia, Dept Med, Augusta, GA 30912 USA

[2] Inst Mol Med & Genet, Augusta, GA USA

来源：

GENESIS | 2002年 / 33卷 / 01期

关键词：

Cre recombinase; TAT; protein transduction; induced DNA recombination; gene deletion;

D O I：

10.1002/gene.10089

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites. genesis 33:48-54, 2002. (C) 2002 Wiley-Liss, Inc.

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页码：48 / 54

页数：7

相关论文

共 13 条

[1] TAT-MEDIATED DELIVERY OF HETEROLOGOUS PROTEINS INTO CELLS [J].

FAWELL, S ;

SEERY, J ;

DAIKH, Y ;

MOORE, C ;

CHEN, LL ;

PEPINSKY, B ;

BARSOUM, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (02) :664-668

[2] CELLULAR UPTAKE OF THE TAT PROTEIN FROM HUMAN IMMUNODEFICIENCY VIRUS [J].

FRANKEL, AD ;

PABO, CO .

CELL, 1988, 55 (06) :1189-1193

[3] AUTONOMOUS FUNCTIONAL DOMAINS OF CHEMICALLY SYNTHESIZED HUMAN IMMUNODEFICIENCY VIRUS TAT TRANS-ACTIVATOR PROTEIN [J].

GREEN, M ;

LOEWENSTEIN, PM .

CELL, 1988, 55 (06) :1179-1188

[4] A conditional null allele of the major histocompatibility IA-beta chain gene [J].

Hashimoto, K ;

Joshi, SK ;

Koni, PA .

GENESIS, 2002, 32 (02) :152-153

[5] Epigenetic regulation of gene structure and function with a cell-permeable Cre recombinase [J].

Jo, DW ;

Nashabi, A ;

Doxsee, C ;

Lin, Q ;

Unutmaz, D ;

Chen, J ;

Ruley, HE .

NATURE BIOTECHNOLOGY, 2001, 19 (10) :929-933

[6] Conditional vascular cell adhesion molecule 1 deletion in mice: Impaired lymphocyte migration to bone marrow [J].

Koni, PA ;

Joshi, SK ;

Temann, UA ;

Olson, D ;

Burkly, L ;

Flavell, RA .

JOURNAL OF EXPERIMENTAL MEDICINE, 2001, 193 (06) :741-753

[7] Transduction of full-length TAT fusion proteins into mammalian cells:: TAT-p27Kip1 induces cell migration [J].

Nagahara, H ;

Vocero-Akbani, AM ;

Snyder, EL ;

Ho, A ;

Latham, DG ;

Lissy, NA ;

Becker-Hapak, M ;

Ezhevsky, SA ;

Dowdy, SF .

NATURE MEDICINE, 1998, 4 (12) :1449-1452

[8]

SAUER B, 1993, METHOD ENZYMOL, V225, P890

[9] Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids [J].

Schmidt, EE ;

Taylor, DS ;

Prigge, JR ;

Barnett, S ;

Capecchi, MR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13702-13707

[10] Protein transduction: unrestricted delivery into all cells? [J].

Schwarze, SR ;

Hruska, KA ;

Dowdy, SF .

TRENDS IN CELL BIOLOGY, 2000, 10 (07) :290-295