Proposal of a five MIRU-VNTR panel to screen clinical isolates of Mycobacterium tuberculosis in Mexico

被引：2

作者：

Bolado-Martinez, Enrique ^{[1
]}

del Carmen Candia-Plata, Maria ^{[2
]}

Zenteno-Cuevas, Roberto ^{[3
]}

Mendoza Damian, Fabiola ^{[4
]}

Aviles-Acosta, Magali ^{[5
]}

Alvarez-Hernandez, Gerardo ^{[2
]}

机构：

[1] Univ Sonora, Dept Ciencias Quim Biol, Hermosillo 83000, Sonora, Mexico

[2] Univ Sonora, Dept Med, Hermosillo 83000, Sonora, Mexico

[3] Univ Veracruzana, Inst Salud Publ, Xalapa 91000, Veracruz, Mexico

[4] Lab Estatal Salud Publ Estado Mexico, Toluca, Mexico

[5] Lab Estatal Salud Publ, Hermosillo, Sonora, Mexico

来源：

ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA | 2015年 / 33卷 / 09期

关键词：

Mycobacterium tuberculosis; MIRU-VNTR; Mexico; GENETIC DIVERSITY; POLYMORPHISM ANALYSIS; COMPLEX; DNA; DIFFERENTIATION;

D O I：

10.1016/j.eimc.2014.10.010

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Introduction: Tuberculosis is a public health problem across Mexico. This paper aims to select a panel, with a minimum number of repetitive elements (MIRU-VNTR) for genotypic characterization of Mycobacterium tuberculosis (M. tuberculosis) clinical isolates. Method: In this study, a full panel of 24 MIRU-VNTR loci was used to discriminate 65 clinical isolates of M. tuberculosis from three different geographical regions of Mexico. Those loci with the highest discriminatory power were subsequently selected. Results: The panel, including five loci, was obtained by selecting the highest values of allelic diversity among the genotypes obtained. The dendrogram, generated by the panel MIRU-VNTR 5, showed a high discriminatory power with 65 unique genotype profiles and formed clusters according to the geographical region of origin. Conclusions: The panel MIRU-VNTR 5 can be useful for characterizing clinical isolates of M. tuberculosis in Mexico. (C) 2014 Elsevier Espana, S.L.U. and Sociedad Espanola de Enfermedades Infecciosas y Microbiologia Clinica. All rights reserved.

引用

页码：609 / 612

页数：4

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