AaPDR3, a PDR Transporter 3, Is Involved in Sesquiterpene β-Caryophyllene Transport in Artemisia annua

被引：58

作者：

Fu, Xueqing ^{[1
]}

Shi, Pu ^{[1
]}

He, Qian ^{[1
]}

Shen, Qian ^{[1
]}

Tang, Yueli ^{[1
]}

Pan, Qifang ^{[1
]}

Ma, Yanan ^{[1
]}

Yan, Tingxiang ^{[1
]}

Chen, Minghui ^{[1
]}

Hao, Xiaolong ^{[1
]}

Liu, Pin ^{[1
]}

Li, Ling ^{[1
]}

Wang, Yuliang ^{[1
]}

Sun, Xiaofen ^{[1
]}

Tang, Kexuan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Joint Int Res Lab Metab & Dev Sci,Sch Agr & Biol, Key Lab Urban Agr South,Minist Agr,Plant Biotechn, Shanghai, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2017年 / 8卷

关键词：

Artemisia annua L; sesquiterpene; ABC transporter; beta-caryophyllene; pleiotropic drug resistance (PDR); transporter; PLEIOTROPIC DRUG-RESISTANCE; BINDING CASSETTE TRANSPORTER; MEMBRANE ABC TRANSPORTER; GLANDULAR TRICHOMES; CONFERS RESISTANCE; NICOTIANA-TABACUM; SYNTHASE; PLANT; EXPRESSION; PROTEIN;

D O I：

10.3389/fpls.2017.00723

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Artemisinin, a sesquiterpenoid endoperoxide, isolated from the plant Artemisia annua L., is widely used in the treatment of malaria. Another sesquiterpenoid, beta-caryophyllene having antibiotic, antioxidant, anticarcinogenic and local anesthetic activities, is also presented in A. annua. The role played by sesquiterpene transporters in trichomes and accumulation of these metabolites is poorly understood in A. annua and in trichomes of other plant species. We identified AaPDR3, encoding a pleiotropic drug resistance (PDR) transporter located to the plasma membrane from A. annua. Expression of AaPDR3 is tissue-specifically and developmentally regulated in A. annua. GUS activity is primarily restricted to T-shaped trichomes of old leaves and roots of transgenic A. annua plants expressing proAaPDR3: GUS. The level of beta-caryophyllene was decreased in transgenic A. annua plants expressing AaPDR3-RNAi while transgenic A. annua plants expressing increased levels of AaPDR3 accumulated higher levels of beta-caryophyllene. When AaPDR3 was expressed in transformed yeast, yeasts expressing AaPDR3 accumulated more beta-caryophyllene, rather than germacrene D and beta-farnesene, compared to the non-expressing control.

引用

页数：12

共 74 条

[1] Isoprenoid biosynthesis in Artemisia annua:: Cloning and heterologous expression of a germacrene A synthase from a glandular trichome cDNA library [J].