Aflp linkage map of an intraspecific cross in Chlamys farreri

被引:0
作者
Wang, S [1 ]
Bao, ZM [1 ]
Pan, B [1 ]
Zhang, LL [1 ]
Yao, B [1 ]
Zhan, AB [1 ]
Bi, K [1 ]
Zhang, QQ [1 ]
机构
[1] Ocean Univ China, Div Life Sci & Technol, Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China
关键词
Chlamys farreri; genetic linkage map; AFLP; intraspecific cross; segregation distortion;
D O I
暂无
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Zhikong scallop, Chlamys farreri, is the most important species for sea-scallop breeding programs in China. The linkage maps of C. farreri were constructed with AFLP markers generated from 21 primer combinations. The polymorphism level of F1 progeny is 62.94%. Of the 783 polymorphic markers detected, 317 segregated in 1:1 ratios and 190 in 3:1 ratios, whereas the other 276 showed segregation distortion (alpha = 0.05). Linkage analysis used the program MAPMAKER/EXP at LOD = 2.5 and 0 = 0.30. The male map containing 94 markers in 19 linkage groups, was 1511.4 cM in total length and 66.56% in genome coverage. The female map containing 97 markers in 20 linkage groups, was 1610.2 cM in total length and 66.05% in genome coverage. The distribution of AFLP markers is relatively even in chromosomes of male and female map, because only few of clusters were observed.
引用
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页码:491 / 499
页数:9
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