Adding new dimensions: towards an integrative understanding of HIV-1 spread

被引：59

作者：

Fackler, Oliver T. ^{[1
,2
]}

Murooka, Thomas T. ^{[3
,4
]}

Imle, Andrea ^{[1
]}

Mempel, Thorsten R. ^{[3
,4
]}

机构：

[1] Univ Heidelberg Hosp, Dept Infect Dis, D-69120 Heidelberg, Germany

[2] Heidelberg Univ, German Ctr Infect Res, D-69120 Heidelberg, Germany

[3] Massachusetts Gen Hosp, CIID, Boston, MA 02114 USA

[4] Harvard Univ, Sch Med, Boston, MA 02115 USA

来源：

NATURE REVIEWS MICROBIOLOGY | 2014年 / 12卷 / 08期

基金：

美国国家卫生研究院;

关键词：

HUMAN-IMMUNODEFICIENCY-VIRUS; T-CELL-ACTIVATION; THYMIC ORGAN-CULTURE; LYMPHOID-TISSUE; NEF INTERFERES; IN-VITRO; B-CELLS; IMMUNOLOGICAL SYNAPSE; SEXUAL TRANSMISSION; SUBCAPSULAR SINUS;

D O I：

10.1038/nrmicro3309

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In vitro studies in primary or immortalized cells continue to be used to elucidate the essential principles that govern the interactions between HIV-1 and isolated target cells. However, until recently, substantial technical barriers prevented this information from being efficiently translated to the more complex scenario of HIV-1 spread in the host in vivo, which has limited our understanding of the impact of host physiological parameters on the spread of HIV-1. In this Review, we discuss the recent development of imaging approaches to visualize HIV-1 spread and the adaptation of these approaches to organotypic ex vivo models and animal models. We focus on new concepts, including the mechanisms and in vivo relevance of cell-cell transmission for HIV-1 spread and the function of the HIV-1 pathogenesis factor Nef, which have emerged from the application of these integrative approaches in complex cell systems.

引用

页码：563 / 574

页数：12

共 132 条

[1] A human colorectal explant culture to evaluate topical microbicides for the prevention of HIV infection [J].