Distribution of Giardia duodenalis genotypes and subgenotypes in raw urban wastewater in Milwaukee, Wisconsin

被引：84

作者：

Sulaiman, IM

Jiang, JL

Singh, A

Xiao, LH

机构：

[1] Ctr Dis Control & Prevent, Div Parasit Dis, Natl Ctr Infect Dis, US PHS,US Dept Hlth & Human Serv, Atlanta, GA 30341 USA

[2] City Milwaukee Publ Hlth Labs, Milwaukee, WI 53202 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 06期

关键词：

D O I：

10.1128/AEM.70.6.3776-3780.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Giardia cysts in 131 raw wastewater samples from Milwaukee, Wis., were genotyped by sequence analysis of the triosephosphate isomerase gene which showed the presence of two distinct genotypes (assemblages A and B) of Giardia duodenalis. Of the 131 samples, 111 belonged to assemblage A, and the remaining samples belonged to assemblage B. A high degree of genetic polymorphism was evident within the assemblage B cluster, with 10 distinct subgenotypes identified, eight of which have not been reported before.

引用

页码：3776 / 3780

页数：5

共 37 条

[1] Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces [J].

Amar, CFL ;

Dear, PH ;

Pedraza-Díaz, S ;

Looker, N ;

Linnane, E ;

McLauchlin, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (02) :446-452

[2] Comparison of the levels of intra-specific genetic variation within Giardia muris and Giardia intestinalis [J].

Andrews, RH ;

Monis, PT ;

Ey, PL ;

Mayrhofer, G .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1998, 28 (08) :1179-1185

[3] Sequence analysis of the β-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples [J].

Cacciò, SA ;

De Giacomo, M ;

Pozio, E .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2002, 32 (08) :1023-1030

[4] Giardia cysts in wastewater treatment plants in Italy [J].

Cacciò, SM ;

De Giacomo, M ;

Aulicino, FA ;

Pozio, E .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (06) :3393-3398

[5] GIARDIA SP - COMPARISON OF ELECTROPHORETIC KARYOTYPES [J].

CAMPBELL, SR ;

VANKEULEN, H ;

ERLANDSEN, SL ;

SENTURIA, JB ;

JARROLL, EL .

EXPERIMENTAL PARASITOLOGY, 1990, 71 (04) :470-482

[6] Genetic homogeneity of axenic isolates of Giardia intestinalis derived from acute and chronically infected individuals in Mexico [J].

Cedillo-Rivera, R ;

Darby, JM ;

Enciso-Moreno, JA ;

Ortega-Pierres, G ;

Ey, PL .

PARASITOLOGY RESEARCH, 2003, 90 (02) :119-123

[7] AXENIC CULTURE AND CHARACTERIZATION OF GIARDIA-ARDEAE FROM THE GREAT BLUE HERON (ARDEA-HERODIAS) [J].

ERLANDSEN, SL ;

BEMRICK, WJ ;

WELLS, CL ;

FEELY, DE ;

KNUDSON, L ;

CAMPBELL, SR ;

VANKEULEN, H ;

JARROLL, EL .

JOURNAL OF PARASITOLOGY, 1990, 76 (05) :717-724

[8] SEM EVIDENCE FOR A NEW SPECIES, GIARDIA-PSITTACI [J].

ERLANDSEN, SL ;

BEMRICK, WJ .

JOURNAL OF PARASITOLOGY, 1987, 73 (03) :623-629

[9] MORPHOLOGY OF THE CYST OF GIARDIA-MICROTI BY LIGHT AND ELECTRON-MICROSCOPY [J].

FEELY, DE .

JOURNAL OF PROTOZOOLOGY, 1988, 35 (01) :52-54

[10]

FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x

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