The lipidome and proteome of microsomes from the methylotrophic yeast Pichia pastoris

被引：27

作者：

Klug, Lisa ^{[1
]}

Tarazona, Pablo ^{[2
]}

Gruber, Clemens ^{[3
,4
]}

Grillitsch, Karlheinz ^{[5
]}

Gasser, Brigitte ^{[3
,6
]}

Troetzmueller, Martin ^{[7
]}

Koefeler, Harald ^{[7
]}

Leitner, Erich ^{[8
]}

Feussner, Ivo ^{[2
]}

Mattanovich, Diethard ^{[3
,6
]}

Altmann, Friedrich ^{[3
,4
]}

Daum, Guenther ^{[1
]}

机构：

[1] Graz Univ Technol, Inst Biochem, A-8010 Graz, Austria

[2] Univ Gottingen, Albrecht Von Haller Inst Plant Sci, Dept Plant Biochem, Gottingen, Germany

[3] Austrian Ctr Ind Biotechnol, Vienna, Austria

[4] BOKU Univ Nat Resources & Life Sci Vienna, Dept Chem, Vienna, Austria

[5] Austrian Ctr Ind Biotechnol, Graz, Austria

[6] BOKU Univ Nat Resources & Life Sci Vienna, Dept Biotechnol, Vienna, Austria

[7] Med Univ, Med Res Ctr, Core Facil Mass Spectrometry, Graz, Austria

[8] Graz Univ Technol, Inst Analyt Chem & Food Chem, A-8010 Graz, Austria

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2014年 / 1841卷 / 02期

基金：

奥地利科学基金会;

关键词：

Pichia pastoris; Endoplasmic reticulum; Proteome; Sphingolipids; Phospholipid; Fatty acid; SODIUM DODECYL-SULFATE; ENDOPLASMIC-RETICULUM; PLASMA-MEMBRANE; PHOSPHOLIPID-SYNTHESIS; SUBCELLULAR MEMBRANES; IDENTIFICATION; PROTEINS; TRIACYLGLYCEROL; CHROMATOGRAPHY; SPHINGOLIPIDS;

D O I：

10.1016/j.bbalip.2013.11.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The methylotrophic yeast Pichia pastoris is a popular yeast expression system for the production of heterologous proteins in biotechnology. Interestingly, cell organelles which play an important role in this process have so far been insufficiently investigated. For this reason, we started a systematic approach to isolate and characterize organelles from P. pastoris. In this study, we present a procedure to isolate microsomal membranes at high purity. These samples represent endoplasmic reticulum (ER) fractions which were subjected to molecular analysis of lipids and proteins. Organelle lipidomics included a detailed analysis of glycerophospholipids, fatty acids, sterols and sphingolipids. The microsomal proteome analyzed by mass spectrometry identified typical proteins of the ER known from other cell types, especially Saccharomyces cerevisiae, but also a number of unassigned gene products. The lipidome and proteome analysis of P. pastoris microsomes are prerequisite for a better understanding of functions of this organelle and for modifying this compartment for biotechnological applications. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：215 / 226

页数：12

共 60 条

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