"Perfect" designer chromosome V and behavior of a ring derivative

被引：253

作者：

Xie, Ze-Xiong ^{[1
,2
]}

Li, Bing-Zhi ^{[1
,2
]}

Mitchell, Leslie A. ^{[3
,4
]}

Wu, Yi ^{[1
,2
]}

Qi, Xin ^{[1
,2
]}

Jin, Zhu ^{[1
,2
]}

Jia, Bin ^{[1
,2
]}

Wang, Xia ^{[1
,2
]}

Zeng, Bo-Xuan ^{[1
,2
]}

Liu, Hui-Min ^{[1
,2
]}

Wu, Xiao-Le ^{[1
,2
]}

Feng, Qi ^{[1
,2
]}

Zhang, Wen-Zheng ^{[1
,2
]}

Liu, Wei ^{[1
,2
]}

Ding, Ming-Zhu ^{[1
,2
]}

Li, Xia ^{[1
,2
]}

Zhao, Guang-Rong ^{[1
,2
]}

Qiao, Jian-Jun ^{[1
,2
]}

Cheng, Jing-Sheng ^{[1
,2
]}

Zhao, Meng ^{[1
,2
]}

Kuang, Zheng ^{[3
,4
]}

Wang, Xuya ^{[3
,4
]}

Martin, J. Andrew ^{[3
,4
]}

Stracquadanio, Giovanni ^{[2
,5
,6
,7
]}

Yang, Kun ^{[5
,6
]}

Bai, Xue ^{[1
,2
]}

Zhao, Juan ^{[1
,2
]}

Hu, Meng-Long ^{[1
,2
]}

Lin, Qiu-Hui ^{[1
,2
]}

Zhang, Wen-Qian ^{[1
,2
]}

Shen, Ming-Hua ^{[1
,2
]}

Chen, Si ^{[1
,2
]}

Su, Wan ^{[1
,2
]}

Wang, En-Xu ^{[1
,2
]}

Guo, Rui ^{[1
,2
]}

Zhai, Fang ^{[1
,2
]}

Guo, Xue-Jiao ^{[1
,2
]}

Du, Hao-Xing ^{[1
,2
]}

Zhu, Jia-Qing ^{[1
,2
]}

Song, Tian-Qing ^{[1
,2
]}

Dai, Jun-Jun ^{[1
,2
]}

Li, Fei-Fei ^{[1
,2
]}

Jiang, Guo-Zhen ^{[1
,2
]}

Han, Shi-Lei ^{[1
,2
]}

Liu, Shi-Yang ^{[1
,2
]}

Yu, Zhi-Chao ^{[1
,2
]}

Yang, Xiao-Na ^{[1
,2
]}

Chen, Ken ^{[1
,2
]}

Hu, Cheng ^{[1
,2
]}

Li, Da-Shuai ^{[1
,2
]}

机构：

[1] Tianjin Univ, Key Lab Syst Bioengn, Minist Educ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China

[2] Tianjin Univ, SynBio Res Platform, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China

[3] NYU, Inst Syst Genet, Langone Med Ctr, 550 1St Ave, New York, NY 10016 USA

[4] NYU, Dept Biochem & Mol Pharmacol, Langone Med Ctr, 550 1St Ave, New York, NY 10016 USA

[5] Johns Hopkins Univ, High Throughput Biol Ctr, Baltimore, MD 21205 USA

[6] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21205 USA

[7] Univ Essex, Sch Comp Sci & Elect Engn, Wivenhoe Pk, Colchester CO4 3SQ, Essex, England

[8] Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3BF, Midlothian, Scotland

[9] BGI Shenzhen, Shenzhen 518083, Peoples R China

[10] James D Watson Inst Genome Sci, Hangzhou 310058, Zhejiang, Peoples R China

来源：

SCIENCE | 2017年 / 355卷 / 6329期

基金：

美国国家科学基金会; 英国生物技术与生命科学研究理事会; 中国国家自然科学基金;

关键词：

GENOME; YEAST;

D O I：

10.1126/science.aaf4704

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis. We designed and de novo synthesized 536,024-base pair chromosome synV in the " Build-A-Genome China" course. We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.

引用

页数：8

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