Genetic profiling of cancer with circulating tumor DNA analysis

被引：23

作者：

Lu, Ling ^{[1
]}

Bi, Junqin ^{[2
]}

Bao, Liming ^{[3
,4
]}

机构：

[1] Fudan Univ, Huashan Hosp, Dept Rheumatol, Shanghai 200032, Peoples R China

[2] Chongqing Med Univ, Childrens Hosp, Ctr Clin Mol Med, Chongqing 400065, Peoples R China

[3] Dartmouth Hitchcock Med Ctr, Dept Pathol & Lab Med, Lebanon, NH 03756 USA

[4] Dartmouth Coll, Geisel Sch Med, 1 Med Ctr Dr, Lebanon, NH 03756 USA

来源：

JOURNAL OF GENETICS AND GENOMICS | 2018年 / 45卷 / 02期

关键词：

ctDNA; Cancer genetics; Genetic profile; Liquid biopsy; Precision therapy; METASTATIC BREAST-CANCER; FREE NUCLEIC-ACIDS; CELL-FREE DNA; ACQUIRED-RESISTANCE; CLINICAL-PRACTICE; PERIPHERAL-BLOOD; DIGITAL PCR; PLASMA; MUTATIONS; EXOSOMES;

D O I：

10.1016/j.jgg.2017.11.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circulating cell-free tumor DNA (ctDNA) in the blood is DNA released from apoptotic, circulating, and living tumor cells. ctDNA is about 140 nt in length and has a half-life of about 1.5 h. ctDNA analysis provides a noninvasive means to assess the genetic profile of cancer in real time. With the advent of molecular technologies, including digital PCR and massively parallel sequencing (MPS), ctDNA analysis has shown promise as a highly sensitive and specific alternative to conventional tissue biopsy in cancer detection, longitudinal monitoring, and precision therapy. This review provides an overview of the latest development in our understanding of the biologic characteristics, detection methodologies, and potential clinical implications of ctDNA, as well as the challenges in translating ctDNA analysis from the research arena to patient care. Copyright (C) 2018, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.

引用

页码：79 / 85

页数：7

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