Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology

被引：2

作者：

Li, L. M. ^{[1
,2
,3
]}

Wei, L. ^{[4
]}

Jiang, H. Y. ^{[1
,2
,3
]}

Zhang, Y. ^{[5
]}

Zhang, X. J. ^{[1
,2
,3
]}

Yuan, L. H. ^{[1
,2
,3
]}

Chen, J. P. ^{[1
,2
,3
]}

机构：

[1] Guangdong Entomol Inst, Guangzhou, Guangdong, Peoples R China

[2] Guangdong Publ Lab Wild Anim Conservat & Utilizat, Guangzhou, Guangdong, Peoples R China

[3] Guangdong Key Lab Integrated Pest Management Agr, Guangzhou, Guangdong, Peoples R China

[4] Lishui Univ, Coll Ecol, Lishui, Peoples R China

[5] Inner Mongolia Agr Univ, Coll Anim Sci, Hohhot, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 03期

关键词：

Microsatellite; Polymorphism; Genetic diversity; Acipenser schrenckii; Next-generation sequencing; LOCI;

D O I：

10.4238/2015.July.14.16

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individuals collected from a sturgeon farm. The results show that the mean number of observed alleles per locus is 6.6 (ranging from 2 to 17). Observed and expected heterozygosity values ranged from 0 to 0.958 and from 0.508 to 0.940, respectively. Not a single locus showed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was observed among any pairwise loci. These highly informative microsatellite markers will be useful for genetic diversity and population structure analyses of A. schrenckii and other species of this genus.

引用

页码：7910 / 7913

页数：4

共 10 条

[1] Thousands of microsatellite loci from the venomous coralsnake Micrurus fulvius and variability of select loci across populations and related species [J].