SIMToolbox: a MATLAB toolbox for structured illumination fluorescence microscopy

被引:61
作者
Krizek, Pavel [1 ]
Lukes, Tomas [2 ,3 ]
Ovesny, Martin [1 ]
Fliegel, Karel [2 ]
Hagen, Guy M. [4 ]
机构
[1] Charles Univ Prague, Fac Med 1, Prague 12800, Czech Republic
[2] Czech Tech Univ, Fac Elect Engn, Dept Radioelect, Prague 16627, Czech Republic
[3] Ecole Polytech Fed Lausanne, Lab Opt Biomed, CH-1015 Lausanne, Switzerland
[4] Univ Colorado, UCCS Ctr BioFrontiers Inst, Colorado Springs, CO 80907 USA
关键词
D O I
10.1093/bioinformatics/btv576
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly graphical interface and designed for processing two-dimensional and three-dimensional data acquired by structured illumination microscopy (SIM). Both optical sectioning and super-resolution applications are supported. The software is also capable of maximum a posteriori probability image estimation (MAP-SIM), an alternative method for reconstruction of structured illumination images. MAP-SIM can potentially reduce reconstruction artifacts, which commonly occur due to refractive index mismatch within the sample and to imperfections in the illumination.
引用
收藏
页码:318 / 320
页数:3
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