Characterization of swine leukocyte antigen alleles and haplotypes on a novel miniature pig line, Microminipig

被引：24

作者：

Ando, A. ^{[1
]}

Imaeda, N. ^{[2
]}

Ohshima, S. ^{[1
]}

Miyamoto, A. ^{[1
]}

Kaneko, N. ^{[3
]}

Takasu, M. ^{[2
]}

Shiina, T. ^{[1
]}

Kulski, J. K. ^{[1
,4
]}

Inoko, H. ^{[1
]}

Kitagawa, H. ^{[2
]}

机构：

[1] Tokai Univ, Sch Med, Div Basic Med Sci & Mol Med, Dept Mol Life Sci, Isehara, Kanagawa 2591193, Japan

[2] Gifu Univ, Fac Appl Biol Sci, Dept Vet Med, Gifu 5011193, Japan

[3] Fuji Micra Inc, Fujinomiya 4180005, Japan

[4] Univ Western Australia, Ctr Forens Sci, Nedlands, WA 6008, Australia

来源：

ANIMAL GENETICS | 2014年 / 45卷 / 06期

基金：

日本学术振兴会;

关键词：

crossover region; genotype; miniature pig; polymorphism; swine leukocyte antigen; SLA CLASS-I; MOLECULAR CHARACTERIZATION; PCR-SSP; SEQUENCE; GENES; NOMENCLATURE; DIVERSITY; SYSTEM; POLYMORPHISM; ASSIGNMENT;

D O I：

10.1111/age.12199

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Microminipigs are extremely small-sized, novel miniature pigs that were recently developed for medical research. The inbred Microminipigs with defined swine leukocyte antigen (SLA) haplotypes are expected to be useful for allo- and xenotransplantation studies and also for association analyses between SLA haplotypes and immunological traits. To establish SLA-defined Microminipig lines, we characterized the polymorphic SLA alleles for three class I (SLA-1, SLA-2 and SLA-3) and two class II (SLA-DRB1 and SLA-DQB1) genes of 14 parental Microminipigs using a high-resolution nucleotide sequence-based typing method. Eleven class I and II haplotypes, including three recombinant haplotypes, were found in the offspring of the parental Microminipigs. Two class I and class II haplotypes, Hp-31.0 (SLA-1*1502-SLA-3*070102-SLA-2*1601) and Hp-0.37 (SLA-DRB1*0701-SLA-DQB1*0502), are novel and have not so far been reported in other pig breeds. Crossover regions were defined by the analysis of 22 microsatellite markers within the SLA class III region of three recombinant haplotypes. The SLA allele and haplotype information of Microminipigs in this study will be useful to establish SLA homozygous lines including three recombinants for transplantation and immunological studies.

引用

页码：791 / 798

页数：8

共 29 条

[1] Rapid assignment of the swine major histocompatibility complex (SLA) class I and II genotypes in Clawn miniature swine using PCR-SSP and PCR-RFLP methods [J].

Ando, A ;

Ota, M ;

Sada, M ;

Katsuyama, Y ;

Goto, R ;

Shigenari, A ;

Kawata, H ;

Anzai, T ;

Iwanaga, T ;

Miyoshi, Y ;

Fujimura, N ;

Inoko, H .

XENOTRANSPLANTATION, 2005, 12 (02) :121-126

[2] Genetic polymorphism of the swine major histocompatibility complex (SLA) class I genes, SLA-1, -2 and -3 [J].

Ando, A ;

Kawata, H ;

Shigenari, A ;

Anzai, T ;

Ota, M ;

Katsuyama, Y ;

Sada, M ;

Goto, R ;

Takeshima, S ;

Aida, Y ;

Iwanaga, T ;

Fujimura, N ;

Suzuki, Y ;

Gojobori, T ;

Inoko, H .

IMMUNOGENETICS, 2003, 55 (09) :583-593

[3] SLA-DRB1 and-DQB1 genotyping by the PCR-SSOP-Luminex method [J].

Ando, A. ;

Shigenari, A. ;

Ota, M. ;

Sada, M. ;

Kawata, H. ;

Azuma, F. ;

Kojima-Shibata, C. ;

Nakajoh, M. ;

Suzuki, K. ;

Uenishi, H. ;

Kulski, J. K. ;

Inoko, H. .

TISSUE ANTIGENS, 2011, 78 (01) :49-55

[4] Microsatellite diversity and crossover regions within homozygous and heterozygous SLA haplotypes of different pig breeds [J].

Ando, Asako ;

Uenishi, Hirohide ;

Kawata, Hisako ;

Tanaka-Matsuda, Maiko ;

Shigenari, Atsuko ;

Flori, Laurence ;

Chardon, Patrick ;

Lunney, Joan K. ;

Kulski, Jerzy K. ;

Inoko, Hidetoshi .

IMMUNOGENETICS, 2008, 60 (07) :399-407

[5]

Chen FX, 2004, CELL MOL IMMUNOL, V1, P212

[6] Novel SLA-DQ alleles and their recombinant molecules in xenogeneic stimulation of human T cells [J].

Chen, FX ;

Xie, J ;

Li, NL ;

Zhou, Y ;

Xin, LJ ;

Chou, KY .

TRANSPLANT IMMUNOLOGY, 2005, 14 (02) :83-89

[7] Molecular characterization of swine leukocyte antigen gene diversity in purebred Pietrain pigs [J].

Essler, Sabine E. ;

Ertl, Werner ;

Deutsch, Julia ;

Ruetgen, Barbara C. ;

Groiss, Sandra ;

Stadler, Maria ;

Wysoudil, Bhuma ;

Gerner, Wilhelm ;

Ho, Chak-Sum ;

Saalmueller, Armin .

ANIMAL GENETICS, 2013, 44 (02) :202-205

[8] Molecular characterization of swine leucocyte antigen class II genes in outbred pig populations [J].

Ho, C. -S. ;

Lunney, J. K. ;

Lee, J. -H. ;

Franzo-Romain, M. H. ;

Martens, G. W. ;

Rowland, R. R. R. ;

Smith, D. M. .

ANIMAL GENETICS, 2010, 41 (04) :428-432

[9] Molecular characterization of swine leucocyte antigen class I genes in outbred pig populations [J].

Ho, C. -S. ;

Lunney, J. K. ;

Franzo-Romain, M. H. ;

Martens, G. W. ;

Lee, Y. -J. ;

Lee, J. -H. ;

Wysocki, M. ;

Rowland, R. R. R. ;

Smith, D. M. .

ANIMAL GENETICS, 2009, 40 (04) :468-478

[10] Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines [J].

Ho, C. S. ;

Franzo-Romain, M. H. ;

Lee, Y. J. ;

Lee, J. H. ;

Smith, D. M. .

INTERNATIONAL JOURNAL OF IMMUNOGENETICS, 2009, 36 (04) :231-234

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