An improved NMR study of liposomes using 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine as model

被引：28

作者：

Cruciani, Oscar

Mannina, Luisa ^{[1
]}

Sobolev, Anatoli P.

Cametti, Cesare

Segre, AnnaLaura

机构：

[1] CNR, Ist Metodol Chim, Monterotondo Stn, I-00016 Rome, Italy

[2] Univ Molise, Dipartimento STAAM, I-86100 Campobasso, Italy

[3] Univ Roma La Sapienza, Dipartimento Fis, I-00185 Rome, Italy

[4] INFM, Unita Roma 1, Rome, Italy

来源：

MOLECULES | 2006年 / 11卷 / 05期

关键词：

liposomes; Unilamellar Vesicles; Multilamellar Vesicles; NMR; HR-MAS; dynamic light scattering;

D O I：

10.3390/11050334

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper we report a comparative characterization of Small Unilamellar Vesicles (SUVs), Large Unilamellar Vesicles (LUVs) and Multilamellar Vesicles (MLVs) prepared from 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine (POPC), carried out using two NMR techniques, namely High Resolution NMR in solution and High Resolution-Magic Angle Spinning (HR-MAS). The size and size distributions of these vesicles were investigated using the dynamic light scattering technique. An improved assignment of the H-1-NMR spectrum of MLVs is also reported.

引用

页码：334 / 344

页数：11

共 33 条

[1] NEGATIVE STAINING OF PHOSPHOLIPIDS + THEIR STRUCTURAL MODIFICATION BY-SURFACE ACTIVE AGENTS AS OBSERVED IN ELECTRON MICROSCOPE [J].