Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay

被引：12

作者：

Vasileiou, Chrysoula ^{[1
]}

Lee, Kin Sing Stephen ^{[1
]}

Crist, Rachael M. ^{[1
]}

Vaezeslami, Soheila ^{[1
]}

Goins, Sarah A. ^{[1
]}

Geiger, James H. ^{[1
]}

Borhan, Babak ^{[1
]}

机构：

[1] Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA

来源：

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 2009年 / 76卷 / 02期

关键词：

electrostatic interactions; hydrophobic effect; ordered water network; carboxylic acid dimer; all-trans-retinoic acid; SITE-DIRECTED MUTANTS; FATTY-ACID; LIGAND-BINDING; HYDROPHOBIC INTERACTIONS; STRUCTURAL-ANALYSIS; CRABP-II; RECEPTORS; CELLS; DIFFERENTIATION; PURIFICATION;

D O I：

10.1002/prot.22334

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by providing a carboxylic acid dimer partner in the form of a Glu residue.

引用

页码：281 / 290

页数：10

共 55 条

[1] Increasing the intracellular availability of all-trans retinoic acid in neuroblastoma cells [J].

Armstrong, JL ;

Ruiz, M ;

Boddy, AV ;

Redfern, CPF ;

Pearson, ADJ ;

Veal, GJ .

BRITISH JOURNAL OF CANCER, 2005, 92 (04) :696-704

[2]

BAILEY JS, 1988, J BIOL CHEM, V263, P9326

[3] Simulations of apo and holo-fatty acid binding protein: Structure and dynamics of protein, ligand and internal water [J].

Bakowies, D ;

van Gunsteren, WF .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 315 (04) :713-736

[4]

BANASZAK L, 1994, ADV PROTEIN CHEM, V45, P89

[5] OVEREXPRESSION OF THE CELLULAR RETINOIC ACID BINDING PROTEIN-I (CRABP-I) RESULTS IN A REDUCTION IN DIFFERENTIATION-SPECIFIC GENE-EXPRESSION IN F9 TERATOCARCINOMA CELLS [J].

BOYLAN, JF ;

GUDAS, LJ .

JOURNAL OF CELL BIOLOGY, 1991, 112 (05) :965-979

[6]

BOYLAN JF, 1992, J BIOL CHEM, V267, P21486

[7] A decade of molecular biology of retinoic acid receptors [J].

Chambon, P .

FASEB JOURNAL, 1996, 10 (09) :940-954

[8] Crystal structure of apo-cellular retinoic acid-binding protein type II (R111M) suggests a mechanism of ligand entry [J].

Chen, X ;

Tordova, M ;

Gilliland, GL ;

Wang, LC ;

Li, Y ;

Yan, HG ;

Ji, XH .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 278 (03) :641-653

[9] Retinoid differentiation therapy in promyelocytic leukemia [J].

Chomienne, C ;

Fenaux, P ;

Degos, L .

FASEB JOURNAL, 1996, 10 (09) :1025-1030

[10] Engineering a rhodopsin protein mimic [J].

Crist, RM ;

Vasileiou, C ;

Rabago-Smith, M ;

Geiger, JH ;

Borhan, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (14) :4522-4523

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