Imiquimod-induced dermatitis impairs thymic tolerance of autoreactive CD4+ T cells to desmoglein 3

被引:4
作者
Iriki, Hisato [1 ]
Mukai, Miho [1 ]
Ito, Hiromi [1 ]
Kurebayashi, Yutaka [2 ]
Amagai, Masayuki [1 ,3 ]
Takahashi, Hayato [1 ]
机构
[1] Keio Univ, Dept Dermatol, Sch Med, Tokyo, Japan
[2] Keio Univ, Dept Pathol, Sch Med, Tokyo, Japan
[3] RIKEN Ctr Integrat Med Sci, Lab Skin Homeostasis, Yokohama, Kanagawa, Japan
关键词
Imiquimod; Immunological tolerance; Thymic tolerance; Central tolerance; Negetive selection; Thymic epitherial cells; Medullary thymic epitherial cells; Cortical thymic epithelial cells; Acute thymic involution; Autoimmunity; Desmoglein; 3; Pemphigus vulgaris; Toll like receptor 7; Toll like receptor 9; DENDRITIC CELLS; PEMPHIGUS-VULGARIS; RECEPTOR; 7; INVOLUTION; INFECTION; DIFFERENTIATION; REPERTOIRE; EXPRESSION; INTERFACE; INDUCTION;
D O I
10.1016/j.jdermsci.2020.09.007
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Background: The thymus plays an essential role in removing autoreactive T cells. Autoantigen-expressing thymic epithelial cells (TECs) contribute to the tolerogenic process. The thymus transiently shrinks as an acute thymic involution (ATI) under various inflammatory conditions. However, whether ATI occurs during local skin inflammation remains unclear, as does its influence on thymic immune tolerance. Objective: We investigated whether imiquimod-induced dermatitis causes ATI and impairs thymic immune tolerance against desmoglein 3 (Dsg3), an epidermal autoantigen of pemphigus vulgaris. Methods: 5% imiquimod cream was applied daily, at 62.5 mg/day (high dose group) or 31.25 mg/day (low dose group), for 1 week on the back of wild-type mice, and to wild-type mice that had undergone bone-marrow transplantation from Dsg3-specific T-cell receptor (TCR) transgenic-Rag2(-/-) mice. Next, thymocytes, TECs and other immune cells were analyzed by flow cytometry. TEC-associated Dsg3 expression was also analyzed by immunofluorescence staining. Results: Thymus weight and thymocyte number in all developmental stages decreased in a dosedependent manner under imiquimod-induced dermatitis. The number of total TECs, specifically medullary, but not cortical, TECs, decreased in high and low dose groups. Accordingly, the number of Dsg3-experssing UEA-1(+)keratin 5(+)mTEC decreased in the thymus during imiquimod-induced dermatitis. Although Dsg3-sepcific transgenic thymocytes was usually deleted in the thymus under physiological condition by central tolerance, Dsg3-sepcific transgenic CD4(+)CD8 thymocytes significantly increased in number under imiquimod-induced dermatitis. Conclusion: These findings indicate a crosstalk between skin and thymus in adult mice and suggest that skin inflammation may impair thymic tolerance to autoantigens, such as Dsg3. (C) 2020 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:166 / 174
页数:9
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