Morpholinos: Antisense and Sensibility

被引：135

作者：

Blum, Martin ^{[1
]}

De Robertis, Edward M. ^{[2
,3
]}

Wallingford, John B. ^{[4
]}

Niehrs, Christof ^{[5
,6
]}

机构：

[1] Univ Hohenheim, Inst Zool, D-70593 Stuttgart, Germany

[2] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90095 USA

[4] Univ Texas Austin, Howard Hughes Med Inst, Dept Mol Biosci, Austin, TX 78712 USA

[5] Inst Mol Biol, D-55128 Mainz, Germany

[6] DKFZ ZMBH Alliance, Div Mol Embryol, D-69120 Heidelberg, Germany

来源：

DEVELOPMENTAL CELL | 2015年 / 35卷 / 02期

关键词：

DOUBLE-STRANDED-RNA; NEURAL CREST; TWISTED GASTRULATION; CELL-MIGRATION; MESSENGER-RNA; ZEBRAFISH; XENOPUS; EMBRYOS; TARGET; SENSE;

D O I：

10.1016/j.devcel.2015.09.017

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

For over 15 years, antisense morpholino oligonucleotides (MOs) have allowed developmental biologists to make key discoveries regarding developmental mechanisms in numerous model organisms. Recently, serious concerns have been raised as to the specificity of MO effects, and it has been recommended to discontinue their usage, despite the long experience of the scientific community with the MO tool in thousands of studies. Reviewing the many advantages afforded by MOs, we conclude that adequately controlled MOs should continue to be accepted as generic loss-of-function approach, as otherwise progress in developmental biology will greatly suffer.

引用

页码：145 / 149

页数：5

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