Anthelmintic Activity of Crude Extract and Essential Oil of Tanacetum vulgare (Asteraceae) against Adult Worms of Schistosoma mansoni

被引：41

作者：

Godinho, Loyana Silva ^{[1
]}

Aleixo de Carvalho, Lara Soares ^{[1
]}

Barbosa de Castro, Clarissa Campos ^{[1
]}

Dias, Mirna Meana ^{[1
]}

Pinto, Priscila de Faria ^{[2
]}

Miller Crotti, Antonio Eduardo ^{[3
]}

Silva Pinto, Pedro Luiz ^{[4
]}

de Moraes, Josue ^{[5
]}

Da Silva Filho, Ademar A. ^{[1
]}

机构：

[1] Univ Fed Juiz de Fora, Fac Farm, Dept Ciencias Farmaceut, BR-36036900 Juiz De Fora, MG, Brazil

[2] Univ Fed Juiz de Fora, Dept Bioquim, Inst Ciencias Biol, BR-36036900 Juiz De Fora, MG, Brazil

[3] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP, Brazil

[4] Adolfo Lutz Inst, Nucleo Enteroparasitas, BR-01246902 Sao Paulo, Brazil

[5] FACIG UNIESP, Fac Ciencias Guarulhos, BR-07025000 Guarulhos, SP, Brazil

来源：

SCIENTIFIC WORLD JOURNAL | 2014年

基金：

巴西圣保罗研究基金会;

关键词：

ANTIMICROBIAL PEPTIDE; CHEMICAL-COMPOSITION; PRAZIQUANTEL; PARTHENOLIDE; DERMASEPTIN; PIPLARTINE; FLOWERS; LEAVES; PLANTS; AMIDE;

D O I：

10.1155/2014/460342

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Schistosomiasis, a parasitic disease caused by trematode flatworms of the genus Schistosoma, affects more than 200 million people worldwide, and its control is dependent on a single drug, praziquantel. Tanacetum vulgare (Asteraceae) is used in folk medicine as a vermifuge. This study aimed to investigate the in vitro schistosomicidal activity of the crude extract (TV) and the essential oil (TV-EO) from the aerial parts of T. vulgare. TV-EO was obtained by hydrodistillation and analyzed by GC/MS, which allowed the identification of beta-thujone (84.13%) as the major constituent. TV and TV-EO, at 200 mu g/mL, decreased motor activity and caused 100% mortality of all adult worms. At 100 and 50 mu g/mL, only TV caused death of all adult worms, while TV-EO was inactive. TV (200 mu g/mL) was also able to reduce viability and decrease production of developed eggs. Confocal laser scanning microscopy showed morphological alterations in the tegument of the S. mansoni surface after incubation with TV (50 and 100 mu g/mL). Quantitative analysis on the schistosomes tegument showed that TV caused changes in the numbers of tubercles of S. mansoni male worms in a dose-dependent manner. The findings suggest that T. vulgare is a potential source of schistosomicidal compounds.

引用

页数：9

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