The effect of magnetic stimulation on unloaded soleus muscle of rat: changes in myosin heavy chain mRNA isoforms

被引：6
作者：
Sakuraba, Tsutomu
Shimada, Yoichi
Takahashi, Shu
Matsunaga, Toshiki
Itoi, Eiji
Kawatani, Masahito
机构：
[1] Akita Univ, Rehabil Div, Sch Med, Akita 0108543, Japan
[2] Akita Univ, Sch Med, Dept Orthopaed Surg, Sect Neuro & Locomotor Sci, Akita 0108543, Japan
[3] Akita Univ, Sch Med, Dept Physiol, Akita 0108543, Japan
来源：
BIOMEDICAL RESEARCH-TOKYO | 2005年 / 26卷 / 01期
关键词：
D O I：
10.2220/biomedres.26.15
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
This study assessed the potential application and the effectiveness of functional magnetic stimulation (FMS) for preventing skeletal muscle atrophy in adult rats. FMS using magnetic stimulator was performed to rat soleus muscle by placing a round magnetic coil on the back of 3(rd)-5(th) lumbar vertebral level at 20 Hz frequency for 60 min/day up to 10 days. A reverse transcriptase-polymerase chain reaction was applied to evaluate relative amounts of mRNAs specific to four myosin heavy chain (MHC) isoforms [MHCI beta, MHCIIa, MHCIIb, and MHClld(x)] in rat soleus muscle during contractile activity by magnetic stimulation. Ten-day unloading by hindlimb suspension induced a drastic decrease of MHCI beta and MHCIIa mRNA expressions, while MHCIIb and MHClld(x) mRNA was not decreased. The magnetic stimulation resuscitated the down-regulation of the mRNA levels of MHCI beta and MHCIIa. These results suggest that magnetic stimulation on acute atrophied muscles is useful for preventing the muscle atrophy.
引用
收藏
页码：15 / 19
页数：5
相关论文
共 27 条
[11]  
Lin V W, 1997, J Spinal Cord Med, V20, P218
[12]   Functional magnetic stimulation of the colon in persons with spinal cord injury [J].
Lin, VW ;
Nino-Murcia, M ;
Frost, F ;
Wolfe, V ;
Hsiao, I ;
Perkash, I .
ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION, 2001, 82 (02) :167-173
[13]   Functional magnetic stimulation for conditioning of expiratory muscles in patients with spinal cord injury [J].
Lin, VW ;
Hsiao, IN ;
Zhu, EC ;
Perkash, I .
ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION, 2001, 82 (02) :162-166
[14]   Functional magnetic stimulation facilitates gastric emptying [J].
Lin, VWH ;
Kim, KH ;
Hsiao, I ;
Brown, W .
ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION, 2002, 83 (06) :806-810
[15]   Functional magnetic stimulation for restoring cough in patients with tetraplegia [J].
Lin, VWH ;
Singh, H ;
Chitkara, RK ;
Perkash, I .
ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION, 1998, 79 (05) :517-522
[16]  
MARSOLAIS EB, 1988, CLIN ORTHOP RELAT R, P64
[17]   FULL-LENGTH RAT ALPHA AND BETA CARDIAC MYOSIN HEAVY-CHAIN SEQUENCES - COMPARISONS SUGGEST A MOLECULAR-BASIS FOR FUNCTIONAL DIFFERENCES [J].
MCNALLY, EM ;
KRAFT, R ;
BRAVOZEHNDER, M ;
TAYLOR, DA ;
LEINWAND, LA .
JOURNAL OF MOLECULAR BIOLOGY, 1989, 210 (03) :665-671
[18]   The effects of therapeutic electric stimulation on acute muscle atrophy in rats after spinal cord injury [J].
Misawa, A ;
Shimada, Y ;
Matsunaga, T ;
Sato, K .
ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION, 2001, 82 (11) :1596-1603
[19]  
Pette D, 1997, Int Rev Cytol, V170, P143, DOI 10.1016/S0074-7696(08)61622-8
[20]   Functional magnetic stimulation of the abdominal muscles in humans [J].
Polkey, MI ;
Luo, YM ;
Guleria, R ;
Hamnegård, CH ;
Green, M ;
Moxham, J .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1999, 160 (02) :513-522
123