Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia

被引:0
作者
Arami, Masoumeh Kourosh [1 ,2 ]
Zade, Javad Mirnajafi [3 ]
Komaki, Alireza [4 ]
Amiri, Mahmood [5 ]
Mehrpooya, Sara [1 ]
Jahanshahi, Ali [3 ]
Jame, Behnam [1 ]
机构
[1] Iran Univ Med Sci, Allied Med Coll, Dept Basic Sci, Tehran, Iran
[2] Saveh Univ Med Sci, Med Coll Saveh, Saveh, Iran
[3] Tarbiat Modares Univ, Dept Physiol, Tehran, Iran
[4] Hamedan Univ Med Sci, Dept Physiol, Hamadan, Iran
[5] Kermanshah Univ Med Sci, Dept Physiol, Kermanshah, Iran
关键词
L-NAME; Nitric oxide; Raphe magnus; Sodium nitroprusside; MEDULLARY RAPHE; TRACTUS-SOLITARII; NEURONS; SYNTHASE; TAIL; THERMOREGULATION; TEMPERATURE; PATHWAY; FEVER;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objective(s): Nucleus Raphe Magnus (NRM) that is involved in the regulation of body temperature contains nitric oxide (NO) synthase. Considering the effect of NO on skin blood flow control, in this study, we assessed its thermoregulatory role within the raphe magnus. Materials and Methods: To this end, tail blood flow of male Wistar rats was measured by laser doppler following the induction of hypothermia. Results: Intra-NRM injection of SNP (exogenous NO donor, 0.1-0.2 mu l, 0.2 nM) increased the blood flow. Similarly, unilateral microinjection of glutamate (0.1-0.2 mu l, 2.3 nM) into the nucleus increased the blood flow. This effect of L-glutamate was reduced by prior intra NRM administration of NO synthase inhibitor N-G-methyl-L-arginine or N-G-nitro-L-arginine methyl ester (L-NAME, 0.1 mu l, 100 nM). Conclusion: It is concluded that NO modulates the thermoregulatory response of NRM to hypothermia and may interact with excitatory amino acids in central skin blood flow regulation.
引用
收藏
页码:989 / 992
页数:4
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