Myelinated mouse nerves studied by X-ray phase contrast zoom tomography

被引：43

作者：

Bartels, M. ^{[1
]}

Krenkel, M. ^{[1
]}

Cloetens, P. ^{[2
]}

Moebius, W. ^{[3
,4
]}

Salditt, T. ^{[1
,4
]}

机构：

[1] Univ Gottingen, Inst Rontgenphys, D-37077 Gottingen, Germany

[2] ESRF European Synchrotron, F-38043 Grenoble, France

[3] Max Planck Inst Expt Med, D-37075 Gottingen, Germany

[4] Ctr Nanoscale Microscopy & Mol Physiol Brain CNMP, Gottingen, Germany

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2015年 / 192卷 / 03期

关键词：

X-ray tomography; Phase contrast; Nerves; Myelin; Node of Ranvier; ELECTRON-MICROSCOPY; INCISURES; SCHMIDT;

D O I：

10.1016/j.jsb.2015.11.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used X-ray phase contrast tomography to resolve the structure of uncut, entire myelinated optic, saphenous and sciatic mouse nerves. Intrinsic electron density contrast suffices to identify axonal structures. Specific myelin labeling by an osmium tetroxide stain enables distinction between axon and surrounding myelin sheath. Utilization of spherical wave illumination enables zooming capabilities which enable imaging of entire sciatic intemodes as well as identification of sub-structures such as nodes of Ranvier and Schmidt-Lanterman incisures. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：561 / 568

页数：8

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