Doxorubicin accumulation in individually electrophoresed organelles

被引：29
作者：
Anderson, AB [1 ]
Xiong, GH [1 ]
Arriaga, EA [1 ]
机构：
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 30期
关键词：
D O I：
10.1021/ja0492539
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the doxorubicin content in individual organelles following their capillary electrophoretic separation and illustrate that chemical accumulation at the subcellular level is highly heterogeneous. In individual mitochondria from cultured human leukemia cells DOX amount is around 50 zmol, 2 orders of magnitude higher than expected from diffusion during drug treatment, and spans 2 orders of magnitude. Copyright © 2004 American Chemical Society.
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收藏
页码：9168 / 9169
页数：2
相关论文
共 12 条
  • [1] A CHEMICAL PERSPECTIVE ON THE ANTHRACYCLINE ANTITUMOR ANTIBIOTICS
    ABDELLA, BRJ
    FISHER, J
    [J]. ENVIRONMENTAL HEALTH PERSPECTIVES, 1985, 64 : 3 - 18
  • [2] Defective acidification in human breast tumor cells and implications for chemotherapy
    Altan, N
    Chen, Y
    Schindler, M
    Simon, SM
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 187 (10) : 1583 - 1598
  • [3] Duffy CF, 2002, ELECTROPHORESIS, V23, P2040, DOI 10.1002/1522-2683(200207)23:13<2040::AID-ELPS2040>3.0.CO
  • [4] 2-3
  • [5] Determination of electrophoretic mobility distributions through the analysis of individual mitochondrial events by capillary electrophoresis with laser-induced fluorescence detection
    Duffy, CF
    Fuller, KM
    Malvey, MW
    O'Kennedy, R
    Arriaga, EA
    [J]. ANALYTICAL CHEMISTRY, 2002, 74 (01) : 171 - 176
  • [6] EVIDENCE OF A COMPLEX BETWEEN ADRIAMYCIN DERIVATIVES AND CARDIOLIPIN - POSSIBLE ROLE IN CARDIOTOXICITY
    GOORMAGHTIGH, E
    CHATELAIN, P
    CASPERS, J
    RUYSSCHAERT, JM
    [J]. BIOCHEMICAL PHARMACOLOGY, 1980, 29 (21) : 3003 - 3010
  • [7] Differences in intracellular sites of action of Adriamycin in neoplastic and normal differentiated cells
    Kiyomiya, K
    Matsuo, S
    Kurebe, M
    [J]. CANCER CHEMOTHERAPY AND PHARMACOLOGY, 2001, 47 (01) : 51 - 56
  • [8] Structural and functional impairment of mitochondria in adriamycin-induced cardiomyopathy in mice:: Suppression of cytochrome c oxidase II gene expression
    Papadopoulou, LC
    Theophilidis, G
    Thomopoulos, GN
    Tsiftsoglou, AS
    [J]. BIOCHEMICAL PHARMACOLOGY, 1999, 57 (05) : 481 - 489
  • [9] Size-dependent electrophoretic migration and separation of liposomes by capillary zone electrophoresis in electrolyte solutions of various ionic strengths
    Radko, SP
    Stastna, M
    Chrambach, A
    [J]. ANALYTICAL CHEMISTRY, 2000, 72 (24) : 5955 - 5960
  • [10] Mitochondrial DNA determines the cellular response to cancer therapeutic agents
    Singh, KK
    Russell, J
    Sigala, B
    Zhang, YG
    Williams, J
    Keshav, KF
    [J]. ONCOGENE, 1999, 18 (48) : 6641 - 6646
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