ETV6-LPXN Fusion Transcript Generated by t(11;12)(q12.1;p13) in a Patient With Relapsing Acute Myeloid Leukemia With NUP98-HOXA9

被引：12

作者：

Abe, Akihiro ^{[1
]}

Yamamoto, Yukiya ^{[1
]}

Iba, Sachiko ^{[1
]}

Kanie, Tadaharu ^{[1
]}

Okamoto, Akinao ^{[1
]}

Tokuda, Masutaka ^{[1
]}

Inaguma, Yoko ^{[1
]}

Yanada, Masamitsu ^{[1
]}

Morishima, Satoko ^{[1
]}

Mizuta, Shuichi ^{[1
]}

Akatsuka, Yoshiki ^{[1
]}

Okamoto, Masataka ^{[1
]}

Kameyama, Toshiki ^{[2
]}

Mayeda, Akila ^{[2
]}

Emi, Nobuhiko ^{[1
]}

机构：

[1] Fujita Hlth Univ, Dept Hematol, Toyoake, Aichi 4701192, Japan

[2] Fujita Hlth Univ, Inst Comprehens Med Sci, Div Gene Express Mech, Toyoake, Aichi 4701192, Japan

来源：

GENES CHROMOSOMES & CANCER | 2016年 / 55卷 / 03期

关键词：

ACUTE MYELOGENOUS LEUKEMIA; PROSTATE-CANCER; TYROSINE PHOSPHORYLATION; FOCAL ADHESION; CELL-LINE; LEUPAXIN; KINASE; RECEPTOR; GENE; PROTEIN;

D O I：

10.1002/gcc.22327

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

ETV6, which encodes an ETS family transcription factor, is frequently rearranged in human leukemias. We show here that a patient with acute myeloid leukemia with t(7;11)(p15;p15) gained, at the time of relapse, t(11;12)(q12.1;p13) with a split ETV6 FISH signal. Using 30-RACE PCR analysis, we found that ETV6 was fused to LPXN at 11q12.1, which encodes leupaxin. ETV6-LPXN, an in-frame fusion between exon 4 of ETV6 and exon 2 of LPXN, did not transform the interleukin-3-dependent 32D myeloid cell line to cytokine independence; however, an enhanced proliferative response was observed when these cells were treated with G-CSF without inhibition of granulocytic differentiation. The 32D and human leukemia cell lines each transduced with ETV6-LPXN showed enhanced migration towards the chemokine CXCL12. We show here for the first time that LPXN is a fusion partner of ETV6 and present evidence indicating that ETV6-LPXN plays a crucial role in leukemia progression through enhancing the response to G-CSF and CXCL12. (C) 2015 Wiley Periodicals, Inc.

引用

页码：242 / 250

页数：9

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